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Expression data from rat insulinoma INS-1 cells under glucotoxic condition

ABSTRACT: Expression data from rat insulinoma INS-1 cells under glucotoxic condition

PROVIDER: PRJNA454198 | ENA |

REPOSITORIES: ENA

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Expression data from rat insulinoma INS-1 cells under glucotoxic condition

Project description:Glucotoxicity of INS-1 cells stimulation by chronic high glucose. We used microarrays to detail the global programme of gene expression underlying cellularization and identified distinct classes of up and down-regulated genes during this process.

2018-05-01 | GSE113828 | GEO

Transcription profiling of rat insulinoma INS-1 cell lines (FLP-In T-REx)

Project description:Four rat insulinoma cell lines (INS-1) were generated that contain a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the cell lines contain the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors or other signal tranduction molecules can be identified. The expression profiles of the parental INS-1 cell line and the four derivatives were determined on a RAE230A array, showing that the highly differentiated phenotype of the parental cell line was maintained in the clones.

2008-06-11 | E-GEOD-1591 | biostudies-arrayexpress

Transcription profiling of rat insulinoma line INS-1 to identify targets of HNF1b mutants in INS-1

Project description:A rat insulinoma cell line (INS-1) was generated that contains a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the line contains the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors HNF1b and HNF1b mutants were identified. Two different clones containing the same transcription factor were analyzed in the non-induced and tet-induced (24 h) state.

2008-06-11 | E-GEOD-1590 | biostudies-arrayexpress

sRNA sequencing of miRNAs exported to HDL from INS-1 rat insulinoma cells

Project description:sRNA sequencing of miRNAs exported to HDL from INS-1 rat insulinoma cells

| PRJNA515308 | ENA

Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

2011-11-22 | GSE31736 | GEO

Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

Project description:Long term exposure to incretin hormones is known to have salutory effects on beta cell function and viability. While short-term cAMP induction is known to have a signature CREB-CRTC target gene response, the long-term effects of cAMP on beta cell gene expression are less well understood. We used rat microarray analysis to compare the genome-wide gene expression response to short-term (2 hours) and long-term (16 hours) stimulations of the cAMP agonist forskolin in INS-1 insulinoma cells. INS-1 cells were exposed to forskolin for 2 or 16 hours in RPMI medium containing 10% serum. Control samples wer incubated for the same time without forskolin. Extracted RNA was used for hybridization on Affymetrix rat 1.0 st gene arrays.

2011-11-22 | E-GEOD-31736 | biostudies-arrayexpress

Transcription profiling of rat insulinoma (INS-1 derived) cell lines that vary in their glucose responsiveness

Project description:The aim of this study was to identify the gene expression changes associated with glucose responsiveness in Rat INS-1 derived cell lines. RNA was prepared from cell lines which were shown to be highly glucose responsive and also lines which were shown to be poorly glucose responsive. Dr. Chris Newgard's lab quantified the RNA and sent it frozen in water. 3 biological replicates per condition were sent for the following conditions: (i) 832/13 and 833/15, robust glucose responsiveness; (ii)832/1 and 832/2 showed poor glucose responsiveness.

2007-09-11 | E-CBIL-35 | biostudies-arrayexpress

INS-1 cell lines (FLP-In T-REx)

2004-11-05 | GSE1591 | GEO

sRNA sequencing of miRNAs exported to HDL from INS-1 rat insulinoma cells

Project description:miRNAs are exported to high density lipoproteins (HDL). This study aimed to understand what miRNAs are exported from INS-1 cells to HDL in vitro.

2019-01-16 | GSE125135 | GEO

RyR2/IRBIT regulates insulin gene transcription, insulin content, and secretion in the insulinoma cell line INS-1

Project description:The role of ER Ca2+ release via ryanodine receptors (RyR) in pancreatic B-cell function is not well defined. Deletion of RyR2 from the rat insulinoma INS-1 enhanced the Ca2+ integral (AUC) stimulated by glucose, and reduced levels of the protein IRBIT (AHCYL1). Deletion of IRBIT increased the Ca2+ AUC in response to glucose via enhanced IP3 receptor activity. Insulin content, basal and glucose-stimulated insulin secretion (GSIS), and INS2 mRNA levels were reduced in both RyR2KO and IRBITKO cells. Nuclear localization of S-adenosylhomocysteinase (AHCY) was increased in RyR2KO and IRBITKO cells, and exon 2 of the INS1 and INS2 genes was more extensively methylated in RyR2KO and IRBITKO cells. Deletion of RyR2 or IRBIT resulted in differential regulation of 314 and 137 proteins, respectively, with 41 in common. We conclude that RyR2 regulates IRBIT levels in INS-1 cells, and together regulate insulin content, GSIS, and the proteome, perhaps via DNA methylation.

2021-11-10 | MSV000088344 | MassIVE

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