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Transcriptomics analysis of human jejunal enteroendocrine cells after vertical sleeve gastrectomy

ABSTRACT: Transcriptomics analysis of human jejunal enteroendocrine cells after vertical sleeve gastrectomy

PROVIDER: PRJNA497450 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8076570.fastq.gz	Fastqsanger.gz
	SRR8076571.fastq.gz	Fastqsanger.gz
	SRR8076573.fastq.gz	Fastqsanger.gz
	SRR8076566.fastq.gz	Fastqsanger.gz
	SRR8076567.fastq.gz	Fastqsanger.gz

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Transcriptomics analysis of enteroendocrine cells after vertical sleeve gastrectomy

Project description:We report here the transcriptome of sorted enteroendocrine cells from duodenum, ileum and colon from mice after vertical sleeve gastrectomy or sham operation, weight matched or not. We analyze the effect of region of origin and surgery and gene expression and found that only region of origin had an impact on EEC transcriptome

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Transcriptomics analysis of enteroendocrine cells following vertical sleeve gastrectomy

Project description:Transcriptomics analysis of enteroendocrine cells following vertical sleeve gastrectomy

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Transcriptomics analysis of human jejunal enteroendocrine cells after vertical sleeve gastrectomy

Project description:We report here the transcriptome of a population enriched in enteroendocrine cells from human jejunum from patients before or after gastrectomy. We analyze the effect of surgery on gene expression and found very little effect on EEC transcriptome

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Peptidomics profiling of the murine gut after vertical sleeve gastrectomy

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Project description:Bariatric surgery, an effective treatment for obesity and diabetes, leads to profound remodeling of whole body energy homeostasis. We utilized a mouse model of vertical sleeve gastrectomy (VSG), a common bariatric surgery as a tool to identify novel secreted proteins and peptides that might act as important metabolic regulators. We analyzed gene expression in the stomach and intestines following VSG or sham surgery in diet-induced obese mice and sought to identify differentially regulated genes encoding secreted proteins/peptides.

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Project description:The gut plays a key role in regulating metabolic health. Dietary factors disrupt intestinal physiology and contribute to obesity and diabetes, whereas bariatric procedures such as vertical sleeve gastrectomy (VSG) cause gut adaptations that induce robust metabolic improvements. However, our understanding of these adaptations at the cellular and molecular levels remains limited. In a validated murine model, we leverage single-cell transcriptomics to determine how VSG impacts different cell lineages of the small intestinal epithelium. We define cell type-specific genes and pathways that VSG rescues from high-fat diet perturbation and characterize additional rescue-independent changes brought about by VSG. We show that Paneth cells have increased expression of the gut peptide Reg3g after VSG. We also find that VSG restores pathways pertaining to mitochondrial respiration and cellular metabolism, especially within crypt-based cells. Overall, our study provides unprecedented molecular resolution of VSG’s therapeutic effects on the gut epithelium.

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