Project description:We have employed microRNA microarray expression profiling as a discovery platform to identify the specific microRNAs that were regulated by circLONP2.
Project description:DLD-1 and MOLT-4 cell lines were cultured in a Rotating cell culture system to simulate microgravity and mRNA expression profile was observed in comparison to Static controls Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line, MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly, mir-22, tumor suppressor microRNA, is highly upregulated in DLD-1.
Project description:We have employed microRNA microarray expression profiling as a discovery platform to identify the specific microRNAs that were regulated by circLONP2.
Project description:Using human colon cancer DLD-1 cells, we engineered a cell line with a doxycycline-inducible single-copy of Snail and compared it to an existing EMT models in DLD-1 where Snail was introduced by episome transfection. Induction of the single-copy line (Snail-lo) with doxycycline increased Snail expression to a level similar to that observed in cancer cell lines spontaneously expressing Snail and results in partial EMT. In comparison, higher levels of overexpression arising from introduction of episomal-Snail (Snail-hi), results in complete EMT.
Project description:In order to elucidate how FOXOs affect diverse cellular processes such as cell cycle progression, stress response and transformation we made use of an inducible version of the FOXO3a protein fused to the hormone binding domain of the human estrogen receptor (FOXO3a-A3-ER) in which all three Akt phosphorylation sites have been mutated to alanine. FOXO3a.A3-ER was stably expressed in the human colon carcinoma cell line DLD-1 (Kops et al., 2002b). In order to analyse the transcriptional response to FOXO3a activation we generated gene expression profiles from DL23 or parental DLD-1 cells, after 6 or 24 hours of 4-OHT treatment using cDNA microarrays.