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Expression profiles of E4BP4 overexpression RAW264.7 cells

ABSTRACT: Expression profiles of E4BP4 overexpression RAW264.7 cells

PROVIDER: PRJNA978599 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24798600.fastq.gz	Fastqsanger.gz
	SRR24798601.fastq.gz	Fastqsanger.gz
	SRR24798596.fastq.gz	Fastqsanger.gz
	SRR24798597.fastq.gz	Fastqsanger.gz
	SRR24798598.fastq.gz	Fastqsanger.gz

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Expression profiles of E4BP4 overexpression RAW264.7 cells

Project description:E4BP4 was overexpressed in RAW264.7 cells using lentivirus and RNAseq was performed.

2024-03-31 | GSE233929 | GEO

Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of E4BP4 knockout (KO) RAW264.7 cells. Methods: We generated E4BP4-KO RAW264.7 Cell Line using CRISPR-CAS9 system. Control plasmid was encoding the Cas9 nuclease without gRNA sequence. Total RNA of E4BP4-KO and Control (CTRL) RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (E4BP4-KO versus CTRL RAW264.7 cells) with three samples each. Results: There were significant differences between E4BP4-KO and CTRL RAW264.7 cells. Conclusions: Deletion of E4BP4 in RAW264.7 cells induced various changes at the transcription level.

2024-03-31 | GSE183076 | GEO

Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

| PRJNA759126 | ENA

Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:ATACseq was performed by overexpressing E4BP4 in RAW264.7 cells using lentivirus.

2024-03-31 | GSE233928 | GEO

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

Project description:ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

| PRJNA759131 | ENA

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

2024-03-31 | GSE183079 | GEO

Expression profiles of CD45+ cells in colon lamina propria from macrophage-specific E4BP4 transgenic mouse

Project description:Purpose: The goals of this study are to confirm the hypothesis that E4BP4 regulates colon specific anti-inflammatory macrophage. Methods: We generated transgenic mice (TG) with macrophage-specific E4BP4 overexpression. CD45+ cells in colon lamina propria were isolated at Zeitgeber Time (ZT) 0 from TG mice and WT littermates during the recovery period of dextran sulfate sodium (DSS)-induced colitis, and total RNA was extracted. CD45+ cells in colon lamina propria RNA profiles were generated by deep sequencing for two groups with one mouse sample each. Results: There were significant differences between TG and WT mice. Conclusions: E4BP4 increased the number of M2 macrophage population and upregulated anti-inflammatory genes.

2024-03-31 | GSE183529 | GEO

Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

| PRJNA978597 | ENA

ChIP-seq for E4BP4 in the liver from transgenic mouse with liver specific E4BP4 overexpression

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of liver-specific E4BP4 overexpression under mouse albumin promoter on the liver glucose and lipid metabolism. Methods: We generated transgenic mice (TG) with liver-specific E4BP4 overexpression, and harvested two livers with one TG mouse each. After cervical dislocation, mouse liver was perfused with a buffer containing Hanks' balanced salt solution, and cross-inked for 30 minutes with 2mM DSG and for 10 minutes with 1% formaldehyde by perfusion, followed by frozen at -80C. Then, Liver was homogenated by gentleMACS Dissociators. Nuclei were isolated and protein-DNA complexes were incubated with antibodies against two kinds of E4BP4 and immunoprecipitated with IgG paramagnetic beads. Results: There were many significant peaks at the promoter/enhancer or intronic region of many genes. Conclusions: E4BP4 possibly regulates the expression of some genes linked to lipid metabolism in the liver.

2021-05-01 | GSE150988 | GEO

Expression profiles of colon macrophages from macrophage-specific E4BP4 transgenic mouse [ColonMac_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of macrophage-specific E4BP4 overexpression under c-fms gene promoter on colon macrophages during the recovery phase of Dextran Sodium Sulfate (DSS)-induced colitis. Methods: We generated transgenic mice (TG) with macrophage-specific E4BP4 overexpression. Colon macrophages were isolated at Zeitgeber Time (ZT) 0 from TG mice and WT littermates and total RNA was extracted. Colon macrophage RNA profiles were generated by deep sequencing for two groups with three mouse samples each. Results: There were significant differences between TG and WT mice. Conclusions: Colon macrophages from the E4BP4 transgenic mice during the recovery phase of DSS-induced colitis might be altered at the transcription level.

2024-03-31 | GSE183075 | GEO

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