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RNA-seq analysis of alleles of the atrR transcription factor-encoding gene in Aspergillus fumigatus

ABSTRACT: RNA-seq analysis of alleles of the atrR transcription factor-encoding gene in Aspergillus fumigatus

PROVIDER: PRJNA508764 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8286620_1.fastq.gz	Fastqsanger.gz
	SRR8286620_2.fastq.gz	Fastqsanger.gz
	SRR8286621_1.fastq.gz	Fastqsanger.gz
	SRR8286621_2.fastq.gz	Fastqsanger.gz
	SRR8286614_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of alleles of the atrR transcription factor-encoding gene in Aspergillus fumigatus

Project description:Determine the transcriptomic response of Aspergillus fumigatus strains to different alleles of atrR

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ChIP-seq analysis of transcription factor AtrR binding across the Aspergillus fumigatus genome

Project description:To determine the genomic binding sites for the Aspergillus fumigatus transcription factor AtrR, we utilized three different strains. One was the wild-type AfS35 which contains a wild-type copy of atrR but lacks any HA tag. The other two are both derived from AfS35 but contain a 3X HA tag replacing the stop codon of atrR. These strains are SPF89 and SPF112, respectively. SPF89 uses the wild-type atrR promoter to drive expression of the epitope-tagged allele while SPF112 uses the much strong hspA promoter to drive production of the same protein.

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RNA-seq analysis of AtrR-regulated gene expression in Aspergillus fumigatus cells in response to voriconazole.

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