Project description:We selected humann intervertebral disc samples to perform proteomics analysis. There were 1 case of grade I , 1 case of grade II, 3 cases of grade Ⅲ and 3 cases of grade Ⅳ according to Pfirrmann classfication. RNA seqencing analysis and single-cell RNA sequencing were integrated with proteomics data to identify the hub genes for intervertebral disc degeneration using bioinformatic method.
Project description:To determine a comprehensive transcriptome sequencing profiling of all immune cell subset in cornea, we performed the single cell RNA-sequencing analysis on sort-purified hematopoietic cells from human corneal limbus