Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of glucose exposures on gene expressions in HepG2 cells

ABSTRACT: Effects of glucose exposures on gene expressions in HepG2 cells

PROVIDER: PRJNA591188 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effects of glucose exposures on gene expressions in HepG2 cells

Project description:SULT1E1, the enzyme that specifically and effectively sulfates estrogens to its inactive forms-sulfated estrogens has been found to be highly induced in high glucose (450 mg/dL) cultured human liver hepatocellular carcinoma HepG2 cells. To confirm the SULT1E1 transcription in response to glucose exposures and to investigate whether glucose signal may alter the other xenobiotic metabolizing enzyme transcripts in HepG2 cells, we have conducted whole genome microarray expression profiling as a platform to identify genes that may response to glucose exposures. HepG2 cells were exposed to low glucose (40 mg/dL) or high glucose (450 mg/dL) DMEM for 48 hours respectively. The xenobiotic metabolic enzyme transcripts such as SULTs, CYPs, UGTs and GSTs were significantly altered in response to glucose exposures.

2020-08-26 | GSE140867 | GEO

Transcription profiling of human hepatoma cell line HepG2 following exposures of three heavy metals arsenic, cadmium and nickel and three carcinogens N-dimethylnitrosoamine (DMN), 12-O-tetradecanoylphorbol-13-acetate (TPA) and tetrachloroethylene (TCE)

Project description:From the result of comparative the gene expression analyses of human hepatoma cell line, HepG2 following exposures of three heavy metals; arsenic, cadmium and nickel and three carcinogens; N-dimethylnitrosoamine (DMN), 12-O-tetradecanoylphorbol-13-acetate (TPA) and tetrachloroethylene (TCE), 31-55% of the genes altered by As, Cd and Ni exposure were overlapped with those by three model carcinogen exposures in our experiments. In particular, three heavy metals shared certain characteristics with TPA and TCE in remarkable up-regulations of the genes associated with progression of cell cycle, which might play a central role in heavy metal carcinogenesis. In addition, this characteristic of gene expressions alteration was counteracted by intracellular accumulation of vitamine C in As-exposed cells but not in Cd- and Ni-exposed cells. These results suggest that the cell proliferative responses are caused by reactive oxygen species mainly in As exposure, while other mechanisms would be involved in these responses in Cd and Ni exposures. Experiment Overall Design: In this study, we examined the gene expression alteration in human hepatoma cell line, HepG2 following exposures of three heavy metals; arsenic, cadmium and nickel and three carcinogens; N-dimethylnitrosoamine (DMN), 12-O-tetradecanoylphorbol-13-acetate (TPA) and tetrachloroethylene (TCE) using DNA microarray with 8795 human genes. Furthermore, we also performed the DNA microarray analyses for the heavy metal exposed-cells that were loaded with vitamine C beforehand to examine the effects of antioxidant molecule to heavy metal exposures.

2008-06-16 | E-GEOD-8865 | biostudies-arrayexpress

iTRAQ-Based Quantitative Proteomic Analysis of Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells

Project description:Viscum coloratum (Kom.) Nakai is a variety of biological activities of medicinal plants, and the active component such as polysaccharides or alkaloids, also proved to have the effect of anti-tumor, anti-virus, anti-radiation, anti-oxidation and anti-infection. In this study, we examined the inhibition of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cells growth in a dose-dependent manner using CCK-8 kit assay, and flow cytometry analysis showed that VCP2 delayed cell cycle in G1 phase and induced apoptosis in HepG2 cells which may due to overexpressed p21Wafl/Cip1 and Cyclin D and decreased expressions of Cyclin E and CDK4, and the upregulated Bad, Smac and Caspase-3 and the downregulated Bcl-XL and XIAP may be one of reasons for inducing apoptosis in VCP2-treated HepG2 cells, but these effects may be not obviously related to Bad. Using iTRAQ and 2D-LC-MSMS, 113 differentially expressed proteins identified in normal and VCP2-treated HepG2 cells. Among them, the expressions of 59 proteins were upregulation, and the expressions of 54 proteins were downregulation. GO, pathways and PPI of differentially expressed proteins further analyzed in polysaccharides-treated HepG2 cells by bioinformatic approach. These findings widen our understanding the anti-tumor mechanisms of mistletoe polysaccharide and provide new clues for screening potent responsive protein to polysaccharides.

2018-10-26 | PXD005323 | Pride

Comparison of Protein Expressions of Drug-Metabolizing Enzymes between Human Liver and the Hepatic Cell Lines HepG2, Hep3B and Huh7 using SWATH and MRM-HR Proteomics

Project description:Human hepatic cell lines have been widely used as an in vitro model for the study of drug metabolism and liver toxicity. However, the validity of this model is still a subject of debate because the expressions of various proteins including drug-metabolizing enzymes (DMEs) in the cell lines can differ significantly from that of human livers. In the present study, we first conducted an untargeted proteomics of the microsomes of the cell lines HepG2, Hep3B, and Huh7 in comparison with human livers using a SWATH method. Furthermore, a targeted proteomic approach, named high-resolution multiple reaction monitoring (MRM-HR), was utilized to compare the expressions of pre-selected DMEs between human livers and the cell lines.

2018-04-18 | PXD008190 | Pride

Effects of KD USF2 on gene expressions in C2C12

Project description:Effects of KD USF2 on gene expressions in C2C12

| PRJNA1021546 | ENA

The effects of knock-out PARP1 on HepG2.

Project description:The effects of knock-out PARP1 on HepG2.

| PRJNA1100538 | ENA

Effects of prenylcysteine oxidase 1 (PCYOX1) silencing on HepG2 cell secretome

Project description:We performed a label free mass spectrometry-based proteomic analysis to investigate the effects of PCYOX1 silencing on the secretome of human HepG2 cells

2021-10-12 | PXD022302 | Pride

Effects of melatonin derivatives on gene expressions in human normal keratincytes

Project description:The aim of this study was to test the effects of melatonin derivatives on gene expressions in human normal keratincytes.

2021-03-26 | GSE147588 | GEO

FXR isoform selective effects on hepatoma cell line HepG2

Project description:FXR isoform selective effects on hepatoma cell line HepG2

| PRJNA552265 | ENA

SWATH proteomics of NMN effects

Project description:Nicotinamide mononucleotide (NMN) has emerged as a promising therapeutic intervention for age-related disorders, including Type 2 Diabetes. In this study, we investigated the effects of NMN treatment on glucose uptake and its underlying mechanisms in mouse tissues (muscle, liver, brain and adipose tissue) and cell lines (HepG2 and C2C12). Through a comprehensive proteomic analysis, we uncovered a series of distinct organ-specific effects that contribute to the observed improvements in glucose metabolism.

2024-06-16 | PXD043257 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data