Proteomics

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ITRAQ-Based Quantitative Proteomic Analysis of Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells


ABSTRACT: Viscum coloratum (Kom.) Nakai is a variety of biological activities of medicinal plants, and the active component such as polysaccharides or alkaloids, also proved to have the effect of anti-tumor, anti-virus, anti-radiation, anti-oxidation and anti-infection. In this study, we examined the inhibition of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cells growth in a dose-dependent manner using CCK-8 kit assay, and flow cytometry analysis showed that VCP2 delayed cell cycle in G1 phase and induced apoptosis in HepG2 cells which may due to overexpressed p21Wafl/Cip1 and Cyclin D and decreased expressions of Cyclin E and CDK4, and the upregulated Bad, Smac and Caspase-3 and the downregulated Bcl-XL and XIAP may be one of reasons for inducing apoptosis in VCP2-treated HepG2 cells, but these effects may be not obviously related to Bad. Using iTRAQ and 2D-LC-MSMS, 113 differentially expressed proteins identified in normal and VCP2-treated HepG2 cells. Among them, the expressions of 59 proteins were upregulation, and the expressions of 54 proteins were downregulation. GO, pathways and PPI of differentially expressed proteins further analyzed in polysaccharides-treated HepG2 cells by bioinformatic approach. These findings widen our understanding the anti-tumor mechanisms of mistletoe polysaccharide and provide new clues for screening potent responsive protein to polysaccharides.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

DISEASE(S): Chronic Bronchitis

SUBMITTER: ZEPENG Yin  

LAB HEAD: Yangyang Chai

PROVIDER: PXD005323 | Pride | 2018-10-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
160120_iTRAQ_DB_01.group Other
160120_iTRAQ_DB_01.mgf Mgf
160120_iTRAQ_DB_01.raw Raw
160120_iTRAQ_DB_02.mgf Mgf
160120_iTRAQ_DB_02.raw Raw
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Publications

iTRAQ-Based Quantitative Proteomic Analysis of the Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells.

Chai Yangyang Y   Zhao Min M  

Scientific reports 20170704 1


Viscum coloratum (Kom.) Nakai is one of active medicinal plants, and its active components, especially polysaccharides, have been shown to exhibit bioactivity. In this study, we examined the effects of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cell growth in a dose-dependent manner by using a CCK-8 assay kit. Flow cytometry analysis showed that VCP2 treatment delayed the cell cycle in the G1 phase and induced apoptosis in HepG2 cells, a result possibly due to the  ...[more]

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