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ABSTRACT: miRNA-seq of goat bronchial epithelial cells infected by Pasteurella multocida

PROVIDER: PRJNA655261 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12389801.fastq.gz	Fastqsanger.gz
	SRR12389805.fastq.gz	Fastqsanger.gz
	SRR12389806.fastq.gz	Fastqsanger.gz
	SRR12389807.fastq.gz	Fastqsanger.gz
	SRR12389808.fastq.gz	Fastqsanger.gz

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Project description:RNA-seq of goat bronchial epithelial cells infected by Pasteurella multocida

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Comparison of the transcriptomes of a Pasteurella multocida VP161 wild-type strain with a Pasteurella multocida VP161 hfq mutant

Project description:Objectives: To determine the transcripts that are differentially expressed in a hfq mutant. Hfq is an RNA chaperone that mediates many interactions between regultory RNAs and their mRNA targets. Analysis of the transcriptomes of the Pasteurella multocida wild-type strain and the Pasteurella multocida hfq mutant will allow for identification of genes controlled by hfq and the sRNAs with which it interacts. Methods: RNA sequencing was employed to determine the transcriptomes of a wild-type Pasteurella multocida strain and a hfq mutant strain. Comparison of these two transcriptomes allows for determination of differentially expressed genes and therefore those genes controlled by Hfq and sRNAs with which it interacts.

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Project description:Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in control (cellulose) and garlic-fed C57BL/6J mice serendipitously infected with Pasteurella multocida and identified acute phase response genes, particularly Lcn2 and Orm2, as the major players in the innate response. Also dieraty garlic suppressed pasteurella infection in C57BL/6J mice.

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A compendium of antibiotic-induced transcription profiles reveals broad regulation of P. multocida virulence genes

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A compendium of antibiotic-induced transcription profiles reveals broad regulation of P. multocida virulence genes

Project description:Treatment of bacteria with antibiotics at or close to the inhibitory concentration leads to specific transcriptional responses often affecting target genes and targets pathways. A dataset of transcriptional profiles (compendium) induced by antibiotics with known mode-of-action (MoA) can be used to gain information on the putative MoA of novel substances with unknown MoAs. We used a Pasteurella multocida microarray to generate a compendium of transcriptional profiles and to obtain information on the putative MoA of a novel antibiotic compound. We also show a strong impact of the bacteriostatic antibiotics on P. multocida virulence gene transcription. Keywords: antibiotica treatment, time course Midlog-grown cultures of P. multocida were treated for 10 or 30 min with 8 different antibiotics and one novel compound (thiazin) at minimal inhibitory concentrations (MICs) and were harvested. Control bacteria were not-treated and harvested at approximately the same optical density an OD578 of ~ 0.5. Total RNA was extracted from these samples and labelled with biotin. P. multocida whole genome transcriptional profiling was performed by hybridization on the custom-made Affymetrix microarray according to the manufacturerâs instructions. The experiments were done in triplicates.

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