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Olfactory stimulation regulates the birth of neurons that express specific odorant receptors

ABSTRACT: Olfactory stimulation regulates the birth of neurons that express specific odorant receptors

PROVIDER: PRJNA660168 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12544337_1.fastq.gz	Fastqsanger.gz
	SRR12544337_2.fastq.gz	Fastqsanger.gz
	SRR12544338_1.fastq.gz	Fastqsanger.gz
	SRR12544338_2.fastq.gz	Fastqsanger.gz
	SRR12544339_1.fastq.gz	Fastqsanger.gz

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Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

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Silencing of odorant receptor gene expression by G protein M-NM-2M-NM-3 signaling ensures the expression of one odorant receptor per olfactory sensory neuron

Project description:Olfactory sensory neurons express just one out of a possible ~1000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein M-NM-2M-NM-3 subunits is both necessary and sufficient to suppress the expression of odorant receptor genes, and likely acts through histone methylation to maintain the silenced odorant receptor genes in transcriptionally inactive heterochromatin. These results provide new insights linking receptor activity with the epigenetic mechanisms responsible for ensuring the expression of one odorant receptor per olfactory sensory neuron. Total 6 samples were analyzed-3 controls & 3 samples

2013-12-16 | E-GEOD-53334 | biostudies-arrayexpress

Silencing of odorant receptor gene expression by G protein βγ signaling ensures the expression of one odorant receptor per olfactory sensory neuron

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TRAP-seq of newly generated olfactory sensory neurons from the open and closed sides of the mouse olfactory epithelium following unilateral naris occlusion

Project description:The objectives of this study were to use unilateral naris occlusion (UNO) in order to characterize the effects of olfactory deprivation on the relative abundance of newly generated olfactory sensory neurons that express specific odorant receptors.

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Olfactory stimulation regulates the birth of neurons that express specific odorant receptors

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-10-07 | GSE157120 | GEO

Olfactory Stimulation Regulates the Birth of Neurons That Express Specific Odorant Receptors.

Project description:In mammals, olfactory sensory neurons (OSNs) are born throughout life, ostensibly solely to replace damaged OSNs. During differentiation, each OSN precursor "chooses," out of hundreds of possibilities, a single odorant receptor (OR) gene, which defines the identity of the mature OSN. The relative neurogenesis rates of the hundreds of distinct OSN "subtypes" are thought to be constant, as they are determined by a stochastic process in which each OR is chosen with a fixed probability. Here, using histological, single-cell, and targeted affinity purification approaches, we show that closing one nostril in mice selectively reduces the number of newly generated OSNs of specific subtypes. Moreover, these reductions depend on an animal's age and/or environment. Stimulation-dependent changes in the number of new OSNs are not attributable to altered rates of cell survival but rather production. Our findings indicate that the relative birth rates of distinct OSN subtypes depend on olfactory experience.

| S-EPMC7569022 | biostudies-literature

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