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MiRNA profiling in mouse peritoneal macrophages from macrophage-specific NFATc3 transgenic mice and their control littermates

ABSTRACT: miRNA profiling in mouse peritoneal macrophages from macrophage-specific NFATc3 transgenic mice and their control littermates

PROVIDER: PRJNA682373 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13191840.fastq.gz	Fastqsanger.gz
	SRR13191841.fastq.gz	Fastqsanger.gz
	SRR13191842.fastq.gz	Fastqsanger.gz
	SRR13191843.fastq.gz	Fastqsanger.gz
	SRR13191844.fastq.gz	Fastqsanger.gz

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miRNA profiling in mouse peritoneal macrophages from macrophage-specific NFATc3 transgenic mice and their control littermates

Project description:We generated NFATc3 macrophage-specific transgenic mice (NFATc3MTG) using the piggyback Transposon. Peritoneal macrophages were isolated from NFATc3MTG mice and their control littermates (wild-type), and the miRNA expression profile was analyzed with deep sequencing.

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Project description:Sphingosine 1-phosphate (S1P) is a lysosphingolipid with anti-atherogenic properties, but mechanisms underlying its effects remain unclear. We here analyzed the consequences of overexpressing the S1P receptor type 1 (S1pr1) on peritoneal macrophage gene expression. To amplify S1pr1 signaling in macrophages, double transgenic mice expressing mouse S1pr1 in mononuclear cells were constructed by crossing two lines. The S1pr1-KI line carries a transgenic cassette in the Rosa 26 locus harboring the mouse S1P1 cDNA and separated from the CAG promoter by a lox-Stop-lox insert. S1pr1-KI mice were then crossed to LysMCre mice expressing Cre recombinase under the control of the lysozyme M promoter, which drives macrophage expression. In the offspring the lox-Stop-lox insert is excised in Cre-expressing cells (macrophages), which induces cDNA expression driven by the CAG promoter. At 12 weeks of age, peritoneal leukocytes were isolated from S1pr1-LysMCre mice and control mice (S1pr1-KI) by peritoneal lavage and seeded in cell culture plates. After 2 h non-adherent cells were removed, total RNA was isolated from the remaining macrophages and subjected to microarray gene expression analysis.

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