Ontology highlight
ABSTRACT:
PROVIDER: PRJNA683630 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13258783.fastq.gz | Fastqsanger.gz | |||
| SRR13258784.fastq.gz | Fastqsanger.gz | |||
| SRR13258785.fastq.gz | Fastqsanger.gz | |||
| SRR13258786.fastq.gz | Fastqsanger.gz | |||
| SRR13258787.fastq.gz | Fastqsanger.gz |
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Project description:We showed that, male E. pela, despite displaying complete metamorphosis similar to holometabolous insects, formed the sister group to hemimetabolous female E. pela and also clustered with Hemiptera. The gene expression profile and Gene Ontology (GO) analysis revealed that the two sexes engaged in distinct developmental programs. In particular, female development appeared to prioritize the expression of genes related to cellular, metabolic, developmental processes, and anatomical structure formation in nymphs. In contrast, male larval development is characterized by the significant down-regulation of genes involved in chitin, the respiratory system, and neurons. The results also suggest that pathways involved in fat metabolism and storage are differently used between the sexes. Retinal mRNA profiles of male prepulae and male pupae generated by deep sequencing, no replicate, using Illumina
2014-12-08 | E-GEOD-63934 | biostudies-arrayexpress
Project description:We showed that, male E. pela, despite displaying complete metamorphosis similar to holometabolous insects, formed the sister group to hemimetabolous female E. pela and also clustered with Hemiptera. The gene expression profile and Gene Ontology (GO) analysis revealed that the two sexes engaged in distinct developmental programs. In particular, female development appeared to prioritize the expression of genes related to cellular, metabolic, developmental processes, and anatomical structure formation in nymphs. In contrast, male larval development is characterized by the significant down-regulation of genes involved in chitin, the respiratory system, and neurons. The results also suggest that pathways involved in fat metabolism and storage are differently used between the sexes.
2014-12-08 | GSE63934 | GEO
Project description:Certain phytophagous insects can induce leaf curling in their host plants that may provide protected and nutrient-rich habitats. However, the mechanisms of this induction remain poorly understood. The cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae), is a serious pest of the cassava and causes leaf curling. To reveal the mechanisms of leaf-curl induction, we first inoculated varying numbers of mealybugs in different locations, namely, the apical meristem and the stem, on cassava seedlings. Second, we performed transcriptome analysis using the total RNA extracted from leaves. The results showed that a single insect was able to induce leaf curling, but the intensity and frequency of the leaf curling were positively correlated with the number of insects. Furthermore, the leaf curling occurred when the mealybugs fed on or close to the apical meristem but not when they fed on the stem. Transcriptome analysis identified a total of 3,931 differentially expressed genes (DEGs) from intact plants and the plants inoculated with mealybugs at different time points. GO analysis of the biological processes revealed that the DEGs contained a series of factors for leaf development of the adaxial–abaxial axis, and auxin biosynthesis and polarity. This suggests that alterations in these functions may cause leaf curling.
2023-06-15 | GSE234712 | GEO
Project description:N-glycosylation is one of the most abundant and conserved protein modifications in eukaryotes. This modification serves various important functions, such as protein folding and cellular attachment, but also modulation of a protein’s function. Recently, it has been shown that N-glycosylation of proteins plays a vital role in insect development and survival, which makes it an interesting target for pest control. Despite the importance of protein N-glycosylation in insects, not much is known about insect N-glycoproteomes. Here, we report on the N-glycoproteomes of three major pest insects spanning different insect orders; Drosophila melanogaster (Diptera), Tribolium castaneum (Coleoptera) and Acyrthosiphon pisum (Hemiptera). The number of identified N-glycosylation sites ranged from 889 in T. castaneum, to 941 in D. melanogaster and 1,338 in A. pisum. Comparison between the different insect species revealed both conserved and species-specific glycoproteins. The functionality of the insect glycoproteins together with the conservation of the N-glycosites throughout evolution are discussed. This information can help in the elaboration of novel pest insect control strategies based on interference in insect glycosylation.
2018-06-18 | PXD008745 | Pride
Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
2019-02-25 | PXD011920 | Pride
Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
2019-02-25 | PXD011976 | Pride