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Single cell RNA-seq data on human innate lymphoid cells II.

ABSTRACT: Single cell RNA-seq data on human innate lymphoid cells II.

PROVIDER: PRJNA685828 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13262137.fastq.gz	Fastqsanger.gz
	SRR13262138.fastq.gz	Fastqsanger.gz
	SRR13262139.fastq.gz	Fastqsanger.gz
	SRR13262140.fastq.gz	Fastqsanger.gz
	SRR13262141.fastq.gz	Fastqsanger.gz

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Single cell RNA-seq data on human innate lymphoid cells II.

Project description:Allergic rhinitis is a prevalent condition affecting up to 25% of the global population. The role of innate lymphoid cells in the pathogenesis of allergic diseases have been previously described, however, a single cell transcriptomic analysis to investigate differences between allergic and non-atopic healthy individuals have yet to be fully investigated. Using a combination of single cell RNA/protein and FACS validation, we provide evidence that there is a predominance and dysregulation of a type 2 response in the ILCs of allergic patients.

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Project description:Purpose: Determine the layer-specific indentities and heterogeneity of innate lymphoid cells in the skin by single cell transcriptomic analysis Methods: Innate lymphoid cells were sorted from epidermis, dermis and subcutis of adult B6 mice and subjected to droplet based single-cell captureing and RNA isolation and library preparation. Results: 3,431, 3,356 and 1,061 innate lymphoid cells from epidermis, dermis, and subcutis were analyzed. Unsupervised clustering of the combined data set performed in Seurat identified 6 distinct ILC clusters. Conclusions: This study reveals skin layer-specific transcriptomic signature of innate lymphoid cells

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Project description:Allergic rhinitis is a prevalent condition affecting up to 25% of the global population. Whilst common pharmacotherapies such as anti-histamines and corticosteroids can act as temporary symptom reliever, it does not work in 20% of patients. In these patients, allergen immunotherapy is recommended. The role of innate lymphoid cells in allergen specific immunotherapy which can result in the induction of tolerance towards the sensitizing allergen remains unclear. Using a combination of single cell RNA/protein and FACS validation, we provide evidence that the competence of ILC2 to produce IL-10 is dysregulated in allergic individuals and can be restored in patients who receive grass pollen immunotherapy. We also identified that this mechanism is associated with modification in retinol metabolic pathway, cytokine-cytokine receptor interaction and JAK-STAT signalling pathways in the ILCs.

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