Project description:To investigate the gene expression changes due to LPC-induced calcific nodule formation in porcine aortic valve interstitial cells (paVICs). We then examined differential gene expression due to treatment with dantrolene, which inhibits calcific nodule formation, with and without LPC after 24 hours of exposure.
Project description:Rat OPCs were incubated in the presence of LPC 18:1 or LPC 18:0 and C13-His. Cultures controls included no treatment and a positive control for the differentiation of OPCs. LPC 18:1 was administered at 10 uM while C13-His was administered at 10 uM. Samples were process for mass spectrometry to identify the incorporation of C13-His in newly synthesized proteins.
