Project description:Human aortic endothelial cells were stimulated by lysophosphatidylcholine (LPC) (10μM) with or without interleukin 35 (IL-35) (10ng/mL) or IL-10 (10ng/mL) for 18 hours. Total RNAs were extracted from samples, then mRNA and non-coding RNAs were enriched by removing rRNA from the total RNA. The library was sequenced by Illumina HiSeq4000 using PE100 strategy and the reads were mapped to the human hg19 reference genome.

2019-10-28 | E-MTAB-6604 | biostudies-arrayexpress

HK-2 cells: control and Substance P (SP)-treated

Project description:NK-1R axis in HK-2 cells by treated HK-2-overexpressed NK-1R cells with or without SP.

2023-03-20 | GSE216743 | GEO

RNA sequencing of paVICs exposed to LPC and dantrolene

Project description:To investigate the gene expression changes due to LPC-induced calcific nodule formation in porcine aortic valve interstitial cells (paVICs). We then examined differential gene expression due to treatment with dantrolene, which inhibits calcific nodule formation, with and without LPC after 24 hours of exposure.

2023-03-30 | GSE227229 | GEO

Patient-derived HK-374 glioblastoma cells treated with radiation and ONC201

Project description:RNASeq results for HK-374 treated with DMSO, radiation, ONC201 or radiation plus ONC201

2020-07-08 | GSE153982 | GEO

Protein expression profile changes of HK-2 cells treated with methylglyoxal and carnosine

Project description:HK-2 cells were treated with MGO, carnosine, or combination of both. Differentially expressed proteins (DEPs) were identified by iTRAQ-based mass spectrum, annotated with Gene Ontology (GO) followed by enrichment analysis of GO items and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.

2020-06-18 | PXD019857 |

LPC-promoted Th1 differentiation

Project description:To evaluate the gene expression profile via RNA-seq after treatment with LPC under Th1 polarizing conditions.

2025-02-14 | GSE166812 | GEO

Microarray analysis of HK-2 cells treated with cisplatin

Project description:Chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, we demonstrated that cisplatin induces cell death in a proximal tubular cell line by suppressing glycolysis- and tricarboxylic acid (TCA)/mitochondria-related genes. HK-2 cells were cultured to confluence in 100mm dishes. Total RNA was extracted (QIAGEN, Valencia, CA, USA), and the concentration in the samples was measured using a Micro UV-Vis fluorescence spectrophotometer (Malcom, Tokyo, JAPAN). Sample of 10ɥg of Total RNA from HK-2 cells were labeled with biotin (3'IVT Labeling Kit, Affymetrix, USA) and hybridized (GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays, Affymetrix). The Microarray platform used was Affymetrix HG U219 Array Strip. The arrays were scanned in a GeneAtlas scanner controlled by GeneAtlas Software (Affymetrix, Sta. Clara, USA). Genes were later filtered according to expression fold change (Affymetrix Expression Console software, Affymetrix) Using Affymetrix GeneAtlas System, we determined the gene expression profiles of HK-2 cells treated with cisplatin. Two replicates were made for each timepoints (control, 6h and 24h).

2015-06-09 | E-GEOD-69644 | biostudies-arrayexpress

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