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Transcriptional changes in HK-2 cells treated with lysophosphatidylcholine (LPC)

ABSTRACT: Transcriptional changes in HK-2 cells treated with lysophosphatidylcholine (LPC)

PROVIDER: PRJNA730413 | ENA |

REPOSITORIES: ENA

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Transcriptional changes in HK-2 cells treated with lysophosphatidylcholine (LPC)

Project description:To confirm gene expression changes upon LPC stimulation of HK-2 cells, we performed microarray analysis.

2021-10-31 | GSE174517 | GEO

RNA-Seq of human aortic endothelial cells with lysophosphatidylcholine, IL-35, and IL-10 against controls

Project description:Human aortic endothelial cells were stimulated by lysophosphatidylcholine (LPC) (10μM) with or without interleukin 35 (IL-35) (10ng/mL) or IL-10 (10ng/mL) for 18 hours. Total RNAs were extracted from samples, then mRNA and non-coding RNAs were enriched by removing rRNA from the total RNA. The library was sequenced by Illumina HiSeq4000 using PE100 strategy and the reads were mapped to the human hg19 reference genome.

2019-10-28 | E-MTAB-6604 | biostudies-arrayexpress

HK-2 cells: control and Substance P (SP)-treated

Project description:NK-1R axis in HK-2 cells by treated HK-2-overexpressed NK-1R cells with or without SP.

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RNA sequencing of paVICs exposed to LPC and dantrolene

Project description:To investigate the gene expression changes due to LPC-induced calcific nodule formation in porcine aortic valve interstitial cells (paVICs). We then examined differential gene expression due to treatment with dantrolene, which inhibits calcific nodule formation, with and without LPC after 24 hours of exposure.

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Patient-derived HK-374 glioblastoma cells treated with radiation and ONC201

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Protein expression profile changes of HK-2 cells treated with methylglyoxal and carnosine

Project description:HK-2 cells were treated with MGO, carnosine, or combination of both. Differentially expressed proteins (DEPs) were identified by iTRAQ-based mass spectrum, annotated with Gene Ontology (GO) followed by enrichment analysis of GO items and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.

2020-06-18 | PXD019857 |

Microarray analysis of HK-2 cells treated with cisplatin

Project description:Chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, we demonstrated that cisplatin induces cell death in a proximal tubular cell line by suppressing glycolysis- and tricarboxylic acid (TCA)/mitochondria-related genes. HK-2 cells were cultured to confluence in 100mm dishes. Total RNA was extracted (QIAGEN, Valencia, CA, USA), and the concentration in the samples was measured using a Micro UV-Vis fluorescence spectrophotometer (Malcom, Tokyo, JAPAN). Sample of 10ɥg of Total RNA from HK-2 cells were labeled with biotin (3'IVT Labeling Kit, Affymetrix, USA) and hybridized (GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays, Affymetrix). The Microarray platform used was Affymetrix HG U219 Array Strip. The arrays were scanned in a GeneAtlas scanner controlled by GeneAtlas Software (Affymetrix, Sta. Clara, USA). Genes were later filtered according to expression fold change (Affymetrix Expression Console software, Affymetrix) Using Affymetrix GeneAtlas System, we determined the gene expression profiles of HK-2 cells treated with cisplatin. Two replicates were made for each timepoints (control, 6h and 24h).

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HK-2 cells: control and Substance P (SP)-treated

Project description:HK-2 cells: control and Substance P (SP)-treated

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OPC Cell Culture with LPC 18:1 or LPC 18:1 (both + C13-His)

Project description:Rat OPCs were incubated in the presence of LPC 18:1 or LPC 18:0 and C13-His. Cultures controls included no treatment and a positive control for the differentiation of OPCs. LPC 18:1 was administered at 10 uM while C13-His was administered at 10 uM. Samples were process for mass spectrometry to identify the incorporation of C13-His in newly synthesized proteins.

2022-02-17 | PXD025177 | Pride

Microarray analysis of differentially expressed genes in bicalein-treated Nasopharyngeal carcinoma (HK-1) cell line

Project description:Baicalein is a bioactive compound that is found in an herbal plant, Oroxylum indicum, grown in Southeast Asia including Thailand. There are several reports investigating on the bioactivities of baicalein including an anticancer activity. However, there is no report on the anticancer activity of the baicalein in nasopharyngeal carcinoma. Thus, the aim of this study is to identify differentially expressed genes (DEGs) that are targets of baicalein in order to reveal cellular pathways and molecular mechanisms of the baicalein in nasopharyngeal carcinoma. In this study, NPC HK-1 cells were used as an NPC representative and treated with 10 and 20 µM baicalein for 24 hours before collecting cells for whole transcriptome profiles. The results revealed that a number of cellular pathways, based on KEGG enrichment analysis, are affected baicalein treatment such as focal adhesion and PI3K-Akt signaling pathway which were also phenotypically related to cell migration and invasion assays. Changes in transcriptome profiles of baicalein-treated HK-1 cells would provide insight on baicalein activity in NPC.

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