Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of female Anopheles gambiae mosquitoes blood fed on mice infected with P. berghei and treated with chloroquine or untreated


ABSTRACT: The effect of chloroquine on mosquitoes transcript abundance was assayed by comparing gene expression between mosquitoes fed on a blood meal containing 50 mg/Kg of chloroquine and those that had fed on a normal blood meal. Pools of 50 midguts were dissected and were hybridize with MMC1 (or 20K) microarrays.

Anopheles gambiae female mosquitoes were blood fed on BALB/c mice infected with P. berghei and intraperitonally pre-treated with 50 mg/kg of chloroquine. As controls, mosquitoes were blood fed on untreated P. berghei infected mice.

Mosquitoes were collected 24 hours post-blood feeding and pools of 50 midguts were dissected and processed for hybridization with MMC1 (or 20K) microarrays.Two different biological experiments were performed for each treatment.

INSTRUMENT(S): Tecan LS 300 scanner

ORGANISM(S): Anopheles gambiae

SUBMITTER: Patrícia Abrantes 

PROVIDER: E-MEXP-1201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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