Project description:Induced ablation of Adam10 in Mx1-Cre x Adam10f/f mice results in T cell-independent Inflammatory alopecia. Mx1-Cre x Adam10f/f mice were crossed to the Rag2KO background. After poly(I:C) injection, mouse skin was dissociated and sorted for CD45-positive cells which were subjected to single-cell RNA sequencing analysis.
Project description:Results showed that Chd7 deficiency delay Cbfb-MYH11 induced leukemia, to explore the mechanism, We also performed microarray analysis on c-Kit+ leukemic cells to determine gene expression differences between Mx1-Cre, Cbfb+/56M and Chd7f/f, Mx1-Cre, Cbfb+/56M leukemic cells.
Project description:Induced abltion of Adam10 in Mx1-Cre x Adam10f/f mice results in T cell-independent Inflammatory alopecia. To determine if the Notch signaling pathway was downstream of Adam10, Mx1-Cre x Rbpjf/f mice were generated. After poly(I:C) injection, mouse skin was dissociated and were sorted for CD45-negative cells which were subjected to single-cell RNA sequencing analysis.We found that the ADAM10-Notch signaling axis in type I/III interferon-responsive upper hair follicles (HF) was crucial for the expression of genes that regulated skin microbiota and protected HFs and its stem cell niche from inflammatory destruction.
Project description:Induced abltion of Adam10 in Mx1-Cre x Adam10f/f mice results in T cell-independent Inflammatory alopecia. To determine if dysbiosis is a prerequisite for the development of alopecia, Mx1-Cre x Adam10f/f mice were treated orally with an antibiotic cocktail pre- and post-induction. 30 days after poly(I:C) injection, mouse skin was dissociated and were sorted for CD45-negative cells which were subjected to single-cell RNA sequencing analysis.We found that the ADAM10-Notch signaling axis in type I/III interferon-responsive upper hair follicles (HF) was crucial for the expression of genes that regulated skin microbiota and protected HFs and its stem cell niche from inflammatory destruction.
Project description:Gene expression profiles from the aortic arch of Ldlr-/-Apob100/100 Mttpflox/flox Mx1-Cre mice at different stages of atherosclerosis development
Project description:In addition to a series of in vitro experiments characterizing the role of Coxsackie-Adenovirus Receptor (CAR) in erythropoiesis (GSE147963), in vivo proof of concecpt was obtained from an Mx1-Cre Cxadrfl/fl conditional knockout mouse model. After poly I:C injection (400µg on 3 consecutive days) to induce Cxadr deletion mice were observed for up to 10 months to study changes in erythropoiesis. After development of anemia, mice were sacrificed and myeloid BM progenitors sorted. RNA of purified populations was used for RNA-seq to study molecular effector mechanisms.
Project description:Gene expression profiles from the aortic arch of Ldlr-/-Apob100/100 Mttpflox/flox Mx1-Cre mice at different stages of atherosclerosis development Total RNAs from the aortic arch were collected at different time points during atherosclerosis development (10, 20, 30, 40, 50, and 60 weeks of age), 4-7 mice per time point.