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Expression profiles of E4BP4 overexpression RAW264.7 cells

ABSTRACT: E4BP4 was overexpressed in RAW264.7 cells using lentivirus and RNAseq was performed.

ORGANISM(S): Mus musculus

PROVIDER: GSE233929 | GEO | 2024/03/31

REPOSITORIES: GEO

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Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:ATACseq was performed by overexpressing E4BP4 in RAW264.7 cells using lentivirus.

2024-03-31 | GSE233928 | GEO

Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of E4BP4 knockout (KO) RAW264.7 cells. Methods: We generated E4BP4-KO RAW264.7 Cell Line using CRISPR-CAS9 system. Control plasmid was encoding the Cas9 nuclease without gRNA sequence. Total RNA of E4BP4-KO and Control (CTRL) RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (E4BP4-KO versus CTRL RAW264.7 cells) with three samples each. Results: There were significant differences between E4BP4-KO and CTRL RAW264.7 cells. Conclusions: Deletion of E4BP4 in RAW264.7 cells induced various changes at the transcription level.

2024-03-31 | GSE183076 | GEO

Expression profiles of E4BP4 overexpression RAW264.7 cells

Project description:Expression profiles of E4BP4 overexpression RAW264.7 cells

| PRJNA978599 | ENA

Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

Project description:Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

| PRJNA978597 | ENA

Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

| PRJNA759126 | ENA

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

Project description:ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

| PRJNA759131 | ENA

ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

2024-03-31 | GSE183079 | GEO

ChIP-seq for E4BP4 in the liver from transgenic mouse with liver specific E4BP4 overexpression

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of liver-specific E4BP4 overexpression under mouse albumin promoter on the liver glucose and lipid metabolism. Methods: We generated transgenic mice (TG) with liver-specific E4BP4 overexpression, and harvested two livers with one TG mouse each. After cervical dislocation, mouse liver was perfused with a buffer containing Hanks' balanced salt solution, and cross-inked for 30 minutes with 2mM DSG and for 10 minutes with 1% formaldehyde by perfusion, followed by frozen at -80C. Then, Liver was homogenated by gentleMACS Dissociators. Nuclei were isolated and protein-DNA complexes were incubated with antibodies against two kinds of E4BP4 and immunoprecipitated with IgG paramagnetic beads. Results: There were many significant peaks at the promoter/enhancer or intronic region of many genes. Conclusions: E4BP4 possibly regulates the expression of some genes linked to lipid metabolism in the liver.

2021-05-01 | GSE150988 | GEO

ChIP-seq for E4BP4 in CD4+ T cell

Project description:Here, using ChIP-seq, we demonstrate that the transcriptional repressor Adenovirus E4 promoter-binding protein (E4BP4) binds directly to the Bcl6 promoter, which a key transcription factor controlling Tfh cell differentiation. By obtaining sequence from chromatin immunoprecipitated DNA of E4BP4 overexpressing CD4+T cells, we generated genome-wide binding gene spectrums of E4BP4. These results reveal that E4BP4 interacts with BCL6 and E4BP4 directly modulated the expression of Bcl6 to reveal the mechanism downstream of E4BP4 that regulates Tfh cell differentiation.

2021-01-19 | GSE121387 | GEO

Expression profiles of colon macrophages from macrophage-specific E4BP4 transgenic mouse [ColonMac_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of macrophage-specific E4BP4 overexpression under c-fms gene promoter on colon macrophages during the recovery phase of Dextran Sodium Sulfate (DSS)-induced colitis. Methods: We generated transgenic mice (TG) with macrophage-specific E4BP4 overexpression. Colon macrophages were isolated at Zeitgeber Time (ZT) 0 from TG mice and WT littermates and total RNA was extracted. Colon macrophage RNA profiles were generated by deep sequencing for two groups with three mouse samples each. Results: There were significant differences between TG and WT mice. Conclusions: Colon macrophages from the E4BP4 transgenic mice during the recovery phase of DSS-induced colitis might be altered at the transcription level.

2024-03-31 | GSE183075 | GEO

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