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Genome-wide maps of 8-oxodG in UV/NAC-treated MCF10A cells.

ABSTRACT: Genome-wide maps of 8-oxodG in UV/NAC-treated MCF10A cells.

PROVIDER: PRJNA798917 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17677052_1.fastq.gz	Fastqsanger.gz
	SRR17677052_2.fastq.gz	Fastqsanger.gz
	SRR17677053_1.fastq.gz	Fastqsanger.gz
	SRR17677053_2.fastq.gz	Fastqsanger.gz
	SRR17677054_1.fastq.gz	Fastqsanger.gz

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Genome-wide maps of 8-oxodG in UV/NAC-treated MCF10A cells.

Project description:8-oxodG accumulation within super-enhancers marks fragile CTCF-mediated chromatin loops.

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Dynamic Maps of UV Damage Formation and Repair for the Human Genome

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Genome-wide repair maps of nucleotide excision repair in Drosophila and S2 cells.

Project description:We report the application of single-molecule-based sequencing technology (XR-seq) for high-throughput profiling of nucleotide excision repair in Drosophila four developmental stages and S2 cells. By obtaining over six billion bases of sequence from UV and cisplatin damage antibodies immunoprecipitated excision DNA, we generated genome-wide nucleotide excision repair maps of Drosophila Embryo, Larva, Pupa , two gender of adults and S2 cells . We find that Drosophila performs transcription-coupled repair (TCR) at all its developmental stages. S2 cell carry out TCR response to both UV and Cisplatin damage. Finally, we show that XPC repair factor is required for both global and transcription-coupled repair in Drosophila. This study provides the mechanism of nucleotide excision repair of Drosophila in vivo and vitro response to UV and Cisplatin damage.

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