Project description:Gene expression in A498 cells infected with shCtrl and shMCM8 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shMCM8 A498 cells. Of the 2415 differentially expressed genes (DEGs) found in shMCM8 and shCtrl A498 cells, 1263 were up-regulated and 1152 were down-regulated based on the threshold of absolute fold change ≥ 2 and FDR < 0.05
Project description:Gene expression in HCT-116 cells infected with shCtrl and shRFWD3 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shRFWD3 HCT 116 cells. Of the 1305 differentially expressed genes (DEGs) found in shRFWD3 and shCtrl HCT 116 cells, 629 were up-regulated and 676 were down-regulated based on the threshold of absolute fold change ≥ 2 and FDR < 0.05
Project description:Gene expression in CAL-27 cells infected with shCtrl and shSCUBE3 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shSCUBE3 CAL-27 cells. Of the 931 differentially expressed genes (DEGs) found in shCtrl and shSCUBE3 CAL-27 cells, 353 were up-regulated and 578 were down-regulated based on the threshold of absolute fold change ≥ 1.3 and FDR < 0.05
Project description:microRNAs are post-transcriptional regulators by degrading the target mRNA. miRNAs can regulate and control a variety of biological processes. We want to use these Affymetrix PrimeView microarrays to find different genes between negative control cells and miR-484 cells.
Project description:Glioblastoma stem cells (GSCs), are at the apex of its cellular hierarchy and contribute to glioblastoma progression and tumor recurrence. Gene expression profiling is useful in determining the genome-wide gene expression changes based on the experimental purpose. In order to interrogate the downstream targets of PTPRZ1, we applied gene expression profiling approach to screen the altered genes that are responsible for the functional phenotype changes. The results will provide a cue for mechanical analysis with potential translational values. PTPRZ1-knocking down glioblastoma stem cells (GSCs) and control GSCs were used in this study. RNA were isolated using Primescript RT Master Mix (Takara). Profiling was established by applying PrimeView Affymetrix Human Gene Expression Array.