Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development of Plasmodium falciparum liver stages in human fetal hepatocyte organoid cultures

ABSTRACT: Development of Plasmodium falciparum liver stages in human fetal hepatocyte organoid cultures

PROVIDER: PRJNA819014 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18453061_1.fastq.gz	Fastqsanger.gz
	SRR18453061_2.fastq.gz	Fastqsanger.gz
	SRR18453062_1.fastq.gz	Fastqsanger.gz
	SRR18453062_2.fastq.gz	Fastqsanger.gz
	SRR18453063_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Development of Plasmodium falciparum liver stages in human fetal hepatocyte organoid cultures

Project description:Plasmodium falciparum (Pf) parasite development in liver represents the initial step of the life-cycle in the human host after a Pf-infected mosquito bite. While an attractive stage for life-cycle interruption, understanding of parasite-hepatocyte interaction is inadequate due to limitations of existing in vitro models. We explore the suitability of hepatocyte organoids (HepOrgs) for Pf-development and show that these cells permitted parasite invasion, differentiation and maturation of different Pf strains. Single-cell messenger RNA sequencing (scRNAseq) of Pf-infected HepOrg cells has identified more than 80 Pf-transcripts upregulated on day 5 post-infection. Transcriptional profile changes are found involving distinct metabolic pathways in hepatocytes with Scavenger Receptor B1 (SR-B1) transcripts highly upregulated. A novel functional involvement in schizont maturation is confirmed in fresh primary hepatocytes. Thus, HepOrgs provide a strong foundation for a versatile in vitro model for Pf liver-stages accommodating basic biological studies and accelerated clinical development of novel tools for malaria control.

2023-08-02 | GSE199239 | GEO

Hormone responsive organoid cultures of human endometrium

Project description:Endometrial gland cultures were established from human non-pregnant endometrium and decidua. We analysed the global gene expression profile of the human endometrial gland organoid cultures to assess the similarity of their molecular signature to the tissue of origin. Organoid cultures established from decidua were also included in the analysis to assess their similarity to endometrial dervied cultures.

2017-04-11 | GSE83281 | GEO

Targeted single-cell RNAseq of tonsil organoid cultures

Project description:Targeted single-cell RNAseq of tonsil organoid cultures

| PRJNA665262 | ENA

Hormone-responsive organoid cultures of human endometrium

Project description:The aim of the experiment was to investigate the effects of stimulation of human endometrial organoids with estrogen (E2) and progesterone (P4) In humans, the endometrium, the uterine mucosal lining, undergoes dynamic changes throughout the menstrual cycle and in pregnancy. We adapted conditions used to establish human adult stem cell-derived organoid cultures to generate 3D cultures of human endometrium. Unlike other mucosal epithelia, the endometrium responds dramatically to ovarian sex hormones, estrogen (E2) and progesterone (P4), which regulate cyclical proliferation and differentiation of endometrial glands with concomitant dynamic temporal and spatial expression of their receptors, ERalpha and PR.

2017-04-11 | GSE94723 | GEO

Targeted single-cell RNAseq of tonsil organoid cultures

Project description:Tonsil organoid cultures were prepared from one donor and stimulated with live-attenuated influenza vaccine (LAIV) or left unstimulated. Cells were harvested on the days described below, total T and B cells were sorted, and cells were sequenced using the BD Rhapsody platform with a targeted set of immune-related genes, BCR, and TCR repertoire analysis.

2020-09-24 | GSE158459 | GEO

Expression data from mouse fetal and adult intestinal organoid cultures and intestinal tissues

Project description:Expression data from mouse fetal and adult intestinal organoid cultures and intestinal tissues

| PRJNA487610 | ENA

Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration

Project description:Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration

| PRJNA693445 | ENA

Gene expression of B cell differentiation in Organoid Cultures

Project description:Gene expression of B cell differentiation in Organoid Cultures

| PRJNA1119504 | ENA

scRNA-seq time course of B cell differentiation in organoid cultures

Project description:To understand how different stimulation conditions impacted the maturation of B cells and formation of plasma cells in an ex vivo organoid culture, we preformed 10X single-cell RNA-seq comparing the CD19+ B cells and CD138+ plasma cells at different time points during the organoid culture. Three stimulation conditions were used including H1N1 influenza antigen alone, R848, H1N1 in combination with the TLR7 agonist R848. Additionally, a final set of organoid cultures were stimulated with H1N1 in combination with the TLR7 agonist R848 and at days 16 and 24 inhibitors to BCL6 and EZH2 were added.\

2024-08-01 | GSE269194 | GEO

Mapping of mitogen and metabolic sensitivity in organoids defines requirements for human hepatocyte growth [scRNA-seq]

Project description:Mechanisms underlying human hepatocyte growth in development and regeneration are incompletely understood. In vitro, human fetal hepatocytes (FH) can be robustly grown as organoids, while adult primary human hepatocyte (PHH) organoids remain difficult to expand, suggesting different growth requirements between fetal and adult hepatocytes. Here, we characterize hepatocyte organoid outgrowth using temporal transcriptomic and phenotypic approaches. FHs initiate reciprocal transcriptional programs involving increased proliferation and repressed lipid metabolism upon initiation of organoid growth. We exploited these insights to design maturation conditions for FH organoids, resulting in acquisition of mature hepatocyte morphological traits and increased expression of functional markers. During PHH organoid outgrowth in the same culture condition, the adult transcriptomes initially mimic the fetal transcriptomic signatures, but PHHs rapidly acquire disbalanced proliferation-lipid metabolism dynamics, resulting in steatosis and halted organoid growth. IL6 supplementation, as emerged from the fetal dataset, and simultaneous activation of the metabolic regulator FXR, prevents steatosis and promotes PHH proliferation, resulting in improved expansion of the derived organoids. Single-cell RNA sequencing analyses reveal preservation of their fetal and adult hepatocyte identities in the respective organoid cultures. Our findings uncover mitogen requirements and metabolic differences determining proliferation of hepatocytes changing from development to adulthood.

2024-04-30 | GSE264261 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data