Project description:Genome wide DNA methylation profiling of 16 GCTB tissue samples, 7 GCTB cell lines, 3 chondroblastomas, 3 aneurysmal bone cysts, 3 non-ossifying fibromas, 2 hyperparathyroidism-associated brown tumours, 5 mesenchymal stem cell samples, 6 osteoblast samples. The Illumina Infinium MethylationEPIC BeadChip arrays were used to obtain DNA methylation profiles across approximately 850,000 CpGs.
Project description:GWAT store most of the TAG in mice, ob/ob mice is an obese mice. Ob/ob/Fsp27-/- mice are lean when compared with ob/ob mice. The GWAT weight was dramatically reduced in ob/ob/Fsp27-/- mice. We next extract the total RNA from the GWAT of ob/ob and ob/ob/Fsp27-/- mice, to perform microaary analysis using Mouse Genome 430 2.0 arrays, Affymetrix. We then analysised the up-regulated and down regulated pathways.
Project description:GWAT store most of the TAG in mice, ob/ob mice is an obese mice. Ob/ob/Fsp27-/- mice are lean when compared with ob/ob mice. The GWAT weight was dramatically reduced in ob/ob/Fsp27-/- mice. We next extract the total RNA from the GWAT of ob/ob and ob/ob/Fsp27-/- mice, to perform microaary analysis using Mouse Genome 430 2.0 arrays, Affymetrix. We then analysised the up-regulated and down regulated pathways. We extract the RNA of GWAT from 4 month old mice and hybridization on Affymetrix microarrays. We then analysis the data.
Project description:ob/ob mice is an obese mice. CIDE family proteins including Cidea, Cideb and Cidec play important role in lipid metabolism. Cidea is mainly expressed in the brown adipose tissue (BAT). Cidec is mainly expressed in the BAT and white adipose tissue (WAT). We generated ob/ob/Cidea-/-/Cidec-/- mice to investigate the phenotype of fat tissue. ob/ob/Cidea-/-/Cidec-/- mice are lean when compared with ob/ob mice. The tissue weight and TAG content of BAT and WAT was extreamly decreased in ob/ob/Cidea-/-/Cidec-/- mice compared with that in ob/ob mice. We next extract the total RNA from the BAT and WAT of ob/ob and ob/ob/Cidea-/-/Cidec-/- mice, to perform microarray analysis using Mouse Gene 1.0 ST array system, Affymetrix. We then analysised the up-regulated and down regulated pathways.
Project description:Comparison of each cell mRNA expression pattern. We generated osteoblasts like cell by direct reprogramming technique from human fibroblast. To confirm dOB gene expression pattern similarity to osteoblasts, we analyzed and compared the gene expression profiles of the samples by cDNA microarry. These analysis revealed that dOB are similar to osteoblats in comparative level to MSC-OB.
Project description:Comparison of each cell mRNA expression pattern. We generated osteoblasts like cell by direct reprogramming technique from human fibroblast. To confirm dOB gene expression pattern similarity to osteoblasts, we analyzed and compared the gene expression profiles of the samples by cDNA microarry. These analysis revealed that dOB are similar to osteoblats in comparative level to MSC-OB. 6 samples.
Project description:We hypothesize that gene expression in the lungs of these differentially-treated mice are divergent thus contributing to the disparity in their phenotypes. More specifically, (1) Effects of Leptin-treatment of ob/ob postnatal mice lungs are known to be volume-dependent from 2 to 10 wks of age, and are independent of the hypometabolism associated with leptin deficiency. ; (2) Leptin is critical to postnatal lung remodeling, particularly related to enlarged alveolar surface area. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between lungs of leptin or saline-treated ob/ob postnatal mice. Keywords: leptin, ob, lung