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Effects of triiodothyronine on human osteoblast-like cells: novel insights from a global transcriptome analysis

ABSTRACT: Effects of triiodothyronine on human osteoblast-like cells: novel insights from a global transcriptome analysis

PROVIDER: PRJNA847172 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19579559_1.fastq.gz	Fastqsanger.gz
	SRR19579559_2.fastq.gz	Fastqsanger.gz
	SRR19579560_1.fastq.gz	Fastqsanger.gz
	SRR19579560_2.fastq.gz	Fastqsanger.gz
	SRR19579561_1.fastq.gz	Fastqsanger.gz

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Effects of triiodothyronine on human osteoblast-like cells: novel insights from a global transcriptome analysis

Project description:In order to investigate, at the mRNA level, the signaling pathways through which triiodothyronine (T3) affects osteoblast function, human mesenchymal stem cells derived from adipose tissue were subjected to a pre-established osteoinduction protocol, resulting in osteoblast-like cells, which were cultured with or without T3. RNA-Seq was performed using Illumina platform, and differential gene expression was assessed with DESeq2. Among differentially expressed genes, enrichment analysis was performed for biological processes against the Gene Ontology Consortium database, using both ClusterProfiler R package and STRING.

2022-06-20 | GSE205678 | GEO

Effects of Triiodothyronine on Human Osteoblast-Like Cells: Novel Insights From a Global Transcriptome Analysis.

Project description:Background: Thyroid hormones play a significant role in bone development and maintenance, with triiodothyronine (T3) particularly being an important modulator of osteoblast differentiation, proliferation, and maintenance. However, details of the biological processes (BPs) and molecular pathways affected by T3 in osteoblasts remain unclear. Methods: To address this issue, primary cultures of human adipose-derived mesenchymal stem cells were subjected to our previously established osteoinduction protocol, and the resultant osteoblast-like cells were treated with 1 nm or 10 nm T3 for 72 h. RNA sequencing (RNA-Seq) was performed using the Illumina platform, and differentially expressed genes (DEGs) were identified from the raw data using Kallisto and DESeq2. Enrichment analysis of DEGs was performed against the Gene Ontology Consortium database for BP terms using the R package clusterProfiler and protein network analysis by STRING. Results: Approximately 16,300 genes were analyzed by RNA-Seq, with 343 DEGs regulated in the 1 nm T3 group and 467 upregulated in the 10 nm T3 group. Several independent BP terms related to bone metabolism were significantly enriched, with a number of genes shared among them (FGFR2, WNT5A, WNT3, ROR2, VEGFA, FBLN1, S1PR1, PRKCZ, TGFB3, and OSR1 for 1nM T3; and FZD1, SMAD6, NOG, NEO1, and ENG for 10 nm T3). An osteoblast-related search in the literature regarding this set of genes suggests that both T3 doses are unfavorable for osteoblast development, mainly hindering BMP and canonical and non-canonical WNT signaling. Conclusions: Therefore, this study provides new directions toward the elucidation of the mechanisms of T3 action on osteoblast metabolism, with potential future implications for the treatment of endocrine-related bone pathologies.

| S-EPMC9248766 | biostudies-literature

Effects of triiodothyronine and/or Irisin on human subcutaneous adipocytes: a global transcriptome analysis

Project description:Effects of triiodothyronine and/or Irisin on human subcutaneous adipocytes: a global transcriptome analysis

| PRJNA902379 | ENA

Effects of triiodothyronine and/or Irisin on human subcutaneous adipocytes: a global transcriptome analysis

Project description:In order to investigate, at the mRNA level, the signaling pathways through which triiodothyronine (T3) and irisin (I) affects Human subcutaneous adipocytes function, human Cultured Human subcutaneous preadipocytes were differentiated into mature adipocytes, which were cultured with T3 and/or Irisin, the control group was without treatment. RNA-Seq was performed using Illumina platform, and differential gene expression was assessed with DESeq2. Among differentially expressed genes, enrichment analysis was performed for biological processes against the Gene Ontology Consortium database, using both ClusterProfiler R package and STRING. Grant 2016/03242-3, São Paulo Research Foundation (FAPESP). Grant 409438/2016, National Council for Scientific and Technological Development (CNPq).

2023-06-28 | GSE218129 | GEO

The global gene expression profile of gain of function miR34c in osteoblast

2012-05-03 | GSE36780 | GEO

Human osteoblast treated with Melphalen

Project description:Global expression profile of human osteoblast treated with melphalen, compared to human osteoblast cells treated with diluent control

2010-09-30 | GSE17839 | GEO

The global gene expression profile of gain of function miR34c in osteoblast

Project description:To examine the unbiased global gene expression of gain of miR34c in committed osteoblast in mouse we have performed expression microarray. This approach will allow us to define the novel targets that are negatively regulated by over-expression of miR34c in osteoblast. Total RNA was isolated from primary calvaria from WT and GOF miR34c mouse (n=3) at the age of P3. WT primary calvarial samples were served as control.

2012-05-02 | E-GEOD-36780 | biostudies-arrayexpress

Human osteoblast treated with recombinant TGF-beta

Project description:Global expression profile of human osteoblast treated with recombinant TGF-beta compared to human osteoblast treated with growth media alone

2010-09-30 | GSE17843 | GEO

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2010-09-30 | E-GEOD-17839 | biostudies-arrayexpress

Identify the effects of miR-19a-3p miRNA mimic overexpression in mouse calvarial osteoblast primary cultures

Project description:We used a primary calvarial osteoblast cell line (CalOB) to transfect either a negative control or miR-19a-3p miRNA mimic to understand the effects on global gene expression using bulk RNA-seq.

2023-06-14 | GSE226402 | GEO

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