Project description:Genome-wide survey reveals the Microsatellite characteristics and phylogenomic relationships of Dictyosoma burgeri (Zoarcales, Perciformes)
Project description:Molecular phylogenomics investigates evolutionary relationships based on genomic data. However, despite genomic sequence conservation, changes in protein interactions can occur relatively rapidly and may cause strong functional diversification. To investigate such functional evolution, we here combine phylogenomics with interaction proteomics. We develop this concept by investigating the molecular evolution of the shelterin complex, which protects telomeres, across 16 vertebrate species from zebrafish to humans covering 450 million years of evolution. Our phylointeractomics screen discovers previously unknown telomere-associated proteins and reveals how homologous proteins undergo functional evolution. For instance, we show that TERF1 evolved as a telomere-binding protein in the common stem lineage of marsupial and placental mammals. Phylointeractomics is a versatile and scalable approach to investigate evolutionary changes in protein function and thus can provide experimental evidence for phylogenomic relationships.
Project description:Strains 2-22 (S. agalactiae ST261 isolated from fish) and A909 (ST7) were grown in TH medium, at 30C and harvested at OD 0.3-0.4. Please note: ST261 and ST7 refer to MLST types commonly used in S.agalactiae as a first approach for phylogenomic relationships (MLST is based on the sequence of 7 genes).
Project description:Strains A909 (ST7 strain isolated from human) and CF01173 (ST7 strain isolated from fish) were grown in TH medium at 37C and harvested at OD 0.3-0.4. Please note: ST7 refers to MLST types commonly used in S.agalactiae as a first approach for phylogenomic relationships (MLST is based on the sequence of 7 genes).
Project description:We presented a genome-wide characterization for H3K9 acetylation (H3K9ac) binding regions in normal temperature and heat-stress conditions via ChIP-seq. The results revealed H3K9ac was an extensive epigenetic modulation in A. japonicus. We further identified differentially acetylated regions (DARs) under heat stress.
Project description:Sequencing of mononucleosomal DNA during asynchronous mitosis in Schizosaccharomyces pombe, Schizosaccharomyces octosporus, Schizosaccharomyces japonicus and Saccharomyces cerevisiae Samples from mononucleosomal DNA from asynchronous mitosis of four species of budding (Saccharomyces cerevisiae W303-1a) and fission yeasts (S. pombe wild type 972h-, S. octosporus CBS1804, S. japonicus var. japonicus ade12- FY53) were sequenced (Illumina Genome Analyzer IIx and HiSeq 2500) using the single read and paired end protocol.