Project description:This study evaluates the transcriptome of transgenic Arabidopsis seedlings expressing the transcription factor TCP14 (AT3G47620) under the UBQ10 promoter.
Project description:This study evaluates the transcriptome of transgenic Arabidopsis seedlings expressing the effector HopBB1 from the bacteria Pseudomonas syringae
Project description:We performed transcriptome and ribosome immunoprecipitation studies of transgenic Arabidopsis (Arabidopsis thaliana) Columbia seeds expressing a His6FLAG-tagged version of the ribosomal large subunit protein L18B (35S:HF-RPL18B)
Project description:In order to explore the regulatory network of plant autophagy, this study generated stable transgenic lines expressing ATG5-TurboID, ATG7-TurboID and ATG11-TurboID. Autophagy was induced by combined treatment with the autophagy inducer AZD8055 and biotin. Proximal proteins of autophagy-related proteins under autophagic conditions were enriched using immunoprecipitation coupled with mass spectrometry (IP-MS), followed by network analysis to identify potential interacting proteins and functional modules. This study provides a richer range of candidate targets for further dissecting the autophagy regulatory network in Arabidopsis thaliana.
Project description:The Arabidopsis PSS1 gene, which encodes a glycine-rich protein, plays a pivotal role in nonhost resistance to the soybean oomycete Phytophthora sojae and the fungal pathogen Fusarium virguliforme. To elucidate the molecular basis of PSS1-mediated immunity, we employed miniTurbo-based proximity labeling in stable transgenic Arabidopsis lines expressing miniTurbo fused to either wild-type PSS1 or its nonfunctional mutant variant, PSS1G119D. Seedlings were challenged with or without P. sojae, and biotinylated proteins were isolated and analyzed by mass spectrometry. The resulting PSS1 interactomes revealed several candidate proteins predominantly localized in the plastid and cytosol. Notably, proteins such as LIN2, ATPMEPCRA, and TGG1 were specifically enriched in wild-type PSS1 lines under P. sojae infection, suggesting their potential involvement in PSS1-mediated nonhost resistance. Our findings offer novel insights into immune protein networks and provide a foundational resource for dissecting nonhost immunity mechanisms in plants
