Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Redifferentiated cardiomyocytes retain residual dedifferentiation signatures and are protected against ischemic injury

ABSTRACT: Redifferentiated cardiomyocytes retain residual dedifferentiation signatures and are protected against ischemic injury

PROVIDER: PRJNA910528 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22587927.fastq.gz	Fastqsanger.gz
	SRR22587928.fastq.gz	Fastqsanger.gz
	SRR22587929.fastq.gz	Fastqsanger.gz
	SRR22587930.fastq.gz	Fastqsanger.gz
	SRR22587931.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 45

Similar Datasets

Redifferentiated cardiomyocytes retain residual dedifferentiation signatures and are protected against ischaemic injury

Project description:Near-complete reversal of ERBB2-driven cardiomyocyte dedifferentiation is driven by the Hippo pathway, restoring contractility whilst long-lasting conferring cardioprotection.

2023-02-01 | PXD038450 | Pride

Redifferentiated cardiomyocytes retain residual dedifferentiation signatures and are protected against ischemic injury.

Project description:Cardiomyocyte proliferation and dedifferentiation have fueled the field of regenerative cardiology in recent years, whereas the reverse process of redifferentiation remains largely unexplored. Redifferentiation is characterized by the restoration of function lost during dedifferentiation. Previously, we showed that ERBB2-mediated heart regeneration has these two distinct phases: transient dedifferentiation and redifferentiation. Here we survey the temporal transcriptomic and proteomic landscape of dedifferentiation-redifferentiation in adult mouse hearts and reveal that well-characterized dedifferentiation features largely return to normal, although elements of residual dedifferentiation remain, even after the contractile function is restored. These hearts appear rejuvenated and show robust resistance to ischemic injury, even 5 months after redifferentiation initiation. Cardiomyocyte redifferentiation is driven by negative feedback signaling and requires LATS1/2 Hippo pathway activity. Our data reveal the importance of cardiomyocyte redifferentiation in functional restoration during regeneration but also protection against future insult, in what could lead to a potential prophylactic treatment against ischemic heart disease for at-risk patients.

| S-EPMC10653068 | biostudies-literature

Redifferentiated cardiomyocytes retain residual dedifferentiation signatures and are protected against ischemic injury

Project description:To investigate the effects of cardiomyocyte redifferentiation and its cardioprotective effects, we performed gene expression profiling analysis using data obtained from RNA-seq of whole hearts that were either WT, or ERBB2 over-expressing, at 3 different timepoints both for sham injury and MI injury.

2023-02-02 | GSE220612 | GEO

Towards minimal residual disease-directed therapy in melanoma

Project description:Many patients with advanced cancers achieve dramatic responses to a panoply of therapeutics, yet retain minimal residual disease (MRD), which ultimately results in relapse. To gain insights into the biology of MRD we applied single-cell RNA-sequencing to malignant cells isolated from BRAF-mutant patient-derived xenograft (PDX) melanoma cohorts exposed to concurrent RAF/MEK-inhibition.

2018-07-18 | GSE116237 | GEO

HIRA regulate Arabidopsis dedifferentiation process

Project description:Plant development is characterised by the capacity to reprogram differentiated cells to initiate asexual reproduction and to produce new organs such as lateral roots. To test whether HIRA could be involved in aspects of developmental reprogramming we studied the effect of HIRA during de-differentiation Arabidopsis root tissue was digested with enzyme to generate protoplast. This process would induce plant cell dedifferentiation. Experiments were performed in WT and hira background to understand how hira affect plant dedifferentiation process.

2014-10-01 | E-GEOD-57044 | biostudies-arrayexpress

Time course analysis of dedifferentiation in porcine follicular granulosa cells

Project description:Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a M-bM-^@M-^Xstem cellM-bM-^@M-^Y-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation of GC using global gene expression analyses. Total RNA was isolated from GCs to DFOG cells at 5 time points during dedifferentiation (cultured in 0day, 1day, 2day, 4day and 7day). Each timepoint was performed in triplicate (ie, biological replicates). Using Affymetrix porcine genome array, we performed microarray time course experiments to analyze gene expression profiles during GC dedifferentiation.

2012-11-21 | E-GEOD-18854 | biostudies-arrayexpress

Transcription factors interfering with dedifferentiation induce direct conversion

Project description:Although transcription factor(TF)s regulate differentiation-related processes, including dedifferentiation and direct conversion, functional interactions between TFs regulating these processes are not well understood. Here we show that TFs preventing dedifferentiation are able to induce direct conversion. Using a neural lineage cell line and a large number of TFs expressed in it, we found a subset of TFs whose overexpression strongly interfered with dedifferentiation triggered by a procedure to induce induced pluripotent stem cells (iPSC), through a maintenance mechanism of the cell-type-specific transcriptional profile. Strikingly, the maintenance activity of the interfering TF set was strong enough to induce the cell line-specific transcriptional profile when overexpressed in a heterologous cell type. In addition, TFs that interfered with dedifferentiation in hepatic lineage cells involved known TFs with induction activity for hepatic lineage cells. Our results suggest that dedifferentiation suppresses a cell-type-specific transcriptional profile, which is primarily maintained by a small subset of TFs capable of inducing direct conversion. We anticipate that this functional correlation might be applicable in various cell types, which may include cancer cells, and might facilitate identification of TFs with induction activity to understand differentiation and tumorigenesis Examination of binding of 3 transcription factors and histone modifications in Neural progenitor like cells.

2013-01-01 | E-GEOD-41252 | biostudies-arrayexpress

RNAseq in protected BAP1 mESc

Project description:RNAseq in protected BAP1 mESc

| PRJNA493016 | ENA

Comparative transcriptome analysis of dedifferentiation in porcine mature adipocytes and follicular granulosa cells

Project description:Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a M-bM-^@M-^Xstem cellM-bM-^@M-^Y-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that mature adipocytes (MA) and follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation mechanism of MA and GC using global gene expression analyses. Using Affymetrix porcine genome array, we compared global gene expression profiles during dedifferentiation to search for particular biological functions in genes of which expression intensities were increased or decreased by MA and GC dedifferentiation.

2011-03-10 | E-GEOD-17264 | biostudies-arrayexpress

Dedifferentiation of Gata6+ epidermal cells

Project description:Dedifferentiation of Gata6+ epidermal cells

| PRJNA731921 | ENA