Project description:To find downstream target of SOC1, we attemted global expression profiles in gain-of-function mutants of SOC1; To find downstream target of SOC1, we attemted global expression profiles in soc1-101D: ; Expt. 1 GSM73643, GSM73646; Expt. 2 GSM73647, GSM73648; Expt. 3 GSM73649, GSM73650; To find downstream target of SOC1, we attemted global expression profiles in soc1-2: GSM73649, GSM73651; To find downstream target of SOC1, we attemted global expression profiles in gain-of-function mutants of SOC1: ; GSM73643, GSM73646, GSM73647, GSM73648, GSM73649, GSM73650 Experiment Overall Design: To more completely identify the downstream targets of SOC1, we attemted to compare the expression profiles of g-o-f mutant of SOC1 with the results of soc1-2 mutant
Project description:To find downstream target of SOC1, we attemted global expression profiles in gain-of-function mutants of SOC1 To find downstream target of SOC1, we attemted global expression profiles in soc1-101D: Expt. 1 GSM73643, GSM73646 Expt. 2 GSM73647, GSM73648 Expt. 3 GSM73649, GSM73650 To find downstream target of SOC1, we attemted global expression profiles in soc1-2: GSM73649, GSM73651 To find downstream target of SOC1, we attemted global expression profiles in gain-of-function mutants of SOC1: GSM73643, GSM73646, GSM73647, GSM73648, GSM73649, GSM73650 Keywords: genetic modification
Project description:MADS-domain transcription factors play pivotal roles in numerous developmental processes in Arabidopsis thaliana. While their involvement in flowering transition and floral development has been extensively examined, their functions in root development remain relatively unexplored. Here, we explored the function and genetic interaction of three MADS-box genes (XAL2, SOC1 and AGL24) in primary root development. Our findings revealed that SOC1 and AGL24, both critical components in flowering transition, redundantly act as repressors of primary root growth as the loss of function of either SOC1 or AGL24 partially recovers the primary root growth, meristem cell number, cell production rate, and the length of fully elongated cells of the short-root mutant xal2-2. Furthermore, we observed that the simultaneous overexpression of AGL24 and SOC1 leads to short-root phenotypes, affecting meristem cell number, cell production rate, fully elongated cell size, but only the overexpression of SOC1 affects distal root stem cell differentiation. Additionally, these genes exhibit distinct modes of transcriptional regulation in roots compared to what has been previously reported for aerial tissues. Moreover, our findings revealed that the expression of certain genes involved in cell differentiation, as well as stress responses, which are either upregulated or downregulated in the xal2-2 mutant, reverted to WT levels in the absence of SOC1 or AGL24.
Project description:The floral transition in Arabidopsis is tightly controlled by complex genetic regulatory networks in response to endogenous and environmental flowering signals. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and SHORT VEGETATIVE PHASE (SVP), two key MADS-domain transcription factors, perceive these signals and function as antagonistic flowering regulators. To understand how they mediate the floral transition, we mapped in vivo binding sites of SOC1 and SVP using chromatin immunoprecipitation followed by hybridization to whole-genome tiling arrays (ChIP-chip). Genes encoding proteins with transcription regulator activity and transcription factor activity were the most enriched groups of genes bound by SOC1 and SVP, indicating their central roles in flowering regulatory networks. In combination with gene expression microarray studies, we further identified the genes whose expression was directly regulated by SOC1 or SVP. Among the common direct targets identified, APETALA2 (AP2)-like genes that repress FT and SOC1 expression were downregulated by SOC1, but upregulated by SVP, revealing a complex feedback regulation among key genes determining the integration of flowering signals. SOC1 regulatory regions were also accessed by SOC1 itself and SVP, suggesting that self-activation and repression by SVP contribute to the regulation of SOC1 expression. In addition, ChIP-chip analysis demonstrated that miR156e and miR172a, which are involved in the regulation of AP2-like genes, were direct targets of SOC1 and SVP, respectively. Taken together, these findings reveal that feedback regulatory loops mediated by SOC1 and SVP are essential components of the gene regulatory networks underpinning the integration of flowering signals during the floral transition. soc1-101D and 35S:SVP ChIPed with SOC1 or SVP polyclonal antibody respectively vs. soc1-2 or svp-41 in Arabidopsis 9-day-old whole seedlings