Project description:Epithelial-to-mesenchymal transition is a complex process that occurs in development and cancer metastasis. We show that EMT plasticity occurs in cancer cells when co-culture with fibroblasts. We used microarrays to detail the global program of gene expression in the H69 small-cell lung carcinoma line when in direct contact with CCD8 fibroblasts.
Project description:Cancer associated fibroblasts (CAFs) have been shown to plays crucial roles in cancer progression. Although increasing evidence demonstrates that CAFs have important roles in modulating the aggressive phenotypes of cancer cells, their effects on the tumor vasculature remain underexplored. We co-cultured TIME human microvascular endothelial cells (MECs) with either primary human ovarian CAFs or normal ovarian fibroblasts (NFs) to evaluate the effects of CAFs on phenotypes of endothelial cells.