ABSTRACT: Testate amoebae (Arcellinida, Amoebozoa) community diversity in New England bogs and fens assessed through lineage-specific amplicon sequencing
Project description:High diversity of testate amoebae (Amoebozoa, Arcellinida) detected by HTS analyses in a New England fen using newly-designed taxon-specific primers
Project description:In this study, we measured growth rate of Netzelia tuberspinifera(basionym Difflugia tuberspinifera) under different temperatures and revealed the molecular mechanisms of temperature adaptation based on the transcriptomics along a temperature gradient (15, 25 and 35 °C). This study can provide a reference for in-depth study on testate amoebae in future researches.
Project description:Endosymbiont bacteria in Arcella species (Amoebozoa, Arcellinida)
| PRJEB45379 | ENA
Project description:Updated Phylogenomic Reconstruction of Arcellinid Testate Amoebae
| PRJNA1032600 | ENA
Project description:Examining the relationship between the testate amoeba Hyalosphenia papilio (Arcellinida, Amoebozoa) and its associated intracellular microalgae using molecular and microscopic methods
| PRJNA761372 | ENA
Project description:the voice of the little giants: Arcellinida testate amoebae in environmental DNA-based bioindication, from taxonomy free to haplotypic level
Project description:enterospheres (FEnS) from six aborted fetal’ small intestines, ranging in gestational age from 11 to 22.5 weeks ), and from adult duodenum AEnS were generated based on previously developed protocols for human organoids cultures . We sought to compare the global gene expression profile of FEnS and AEnS by whole transcriptome shotgun sequencing (WTSS) analysis. We evaluated FEnS (N=6) and AEnS (N=3) samples at a low passage, ranging from P6 to P14.
Project description:Peatlands of the Lehstenbach catchment (Germany) house so far unidentified microorganisms with phylogenetically novel variants of the dissimilatory (bi)sulfite reductase genes dsrAB. These genes are characteristic for microorganisms that reduce sulfate, sulfite, or some organosulfonates for energy conservation, but can also be present in anaerobic syntrophs. However, nothing is currently known regarding the abundance, community dynamics, and biogeography of these dsrAB-carrying microorganisms in peatlands. To tackle these issues, soils from a Lehstenbach catchment site (Schlöppnerbrunnen II fen) from different depths were sampled at three time points over a six-year period to analyze the diversity and distribution of dsrAB-containing microorganisms by a newly developed functional gene microarray and quantitative PCR assays. Members of novel, uncultivated dsrAB lineages (approximately representing species-level groups) (i) dominated a temporally stable but spatially structured dsrAB community and (ii) represented ‘core’ members (up to 1-1.7% relative abundance) of the autochthonous microbial community in this fen. In addition, denaturing gradient gel electrophoresis (DGGE)- and clone library-based comparison of the dsrAB diversity in soils from a wet meadow, three bogs, and five fens of various geographic locations (distance ~1-400 km), identified one Syntrophobacter-related and nine novel dsrAB lineages to be widespread in low-sulfate peatlands. Signatures of biogeography in dsrB-DGGE data were not correlated with geographic distance but could largely be explained by soil pH and wetland type, implying that distribution of dsrAB-carrying microorganisms in wetlands on the scale of a few hundred kilometers is not limited by dispersal but determined by contemporary environmental conditions. 36 dsrAB clones for chip evaluation, 33 hybridizations of labeled dsrAB RNA from environmental peatsoil samples
