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ChIP-seq analysis of FfmA binding in Aspergillus fumigatus

ABSTRACT: ChIP-seq analysis of FfmA binding in Aspergillus fumigatus

PROVIDER: PRJNA956572 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24199647_1.fastq.gz	Fastqsanger.gz
	SRR24199647_2.fastq.gz	Fastqsanger.gz
	SRR24199648_1.fastq.gz	Fastqsanger.gz
	SRR24199648_2.fastq.gz	Fastqsanger.gz
	SRR24199649_1.fastq.gz	Fastqsanger.gz

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ChIP-seq analysis of FfmA binding in Aspergillus fumigatus

Project description:Two strains were analyzed by ChIP-seq to determine the genomic binding sites for the Aspergillus fumigatus transcription factor FfmA. A wild-type strain (AfS35) was subjected to ChIP-seq analysis using an antibody directed against the wild-type version of the FfmA protein. An strain containing an epitope-tagged version (FLAG-ffmA) of the ffmA gene under control of a doxycycline off (dox off) promoter was also analyzed by ChIP-seq. In this latter case, an anti-FLAG antibody was used. The negative controls in both cases consisted of a ChIP-seq reaction with the respective primary antibody omitted.

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