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NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days

ABSTRACT: NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days

PROVIDER: PRJNA960846 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24273416_1.fastq.gz	Fastqsanger.gz
	SRR24273416_2.fastq.gz	Fastqsanger.gz
	SRR24273417_1.fastq.gz	Fastqsanger.gz
	SRR24273417_2.fastq.gz	Fastqsanger.gz
	SRR24273418_1.fastq.gz	Fastqsanger.gz

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NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days

Project description:NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days

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Project description:Nontypeable Haemophilus influenzae (NTHi) is a pathobiont which chronically colonises the airway of individuals with chronic respiratory disease. It is unclear how NTHi persists in the airway, however accumulating evidence suggests that NTHi can invade and persist within macrophages. To better understand the mechanisms of NTHi persistence within macrophages, this work developed an in vitro model of NTHi intracellular persistence using human monocyte-derived macrophages (MDM). Dual RNA Sequencing was used to assess MDM and NTHi transcriptomic regulation occurring simultaneously during NTHi persistence. This work demonstrates that NTHi can invade and persist within human macrophages. Macrophages activate innate immune responses, whereas NTHi persistence was facilitated by transcriptomic adaptations in bacterial metabolic, stress response and ribosome pathways. This research provides transcriptomic insights into NTHi-macrophage interactions, enhances our understanding of how NTHi can utilise host immune cells to chronically colonise the airway and identifies potential bacterial gene pathways that may be attractive therapeutic targets

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Transcriptome analysis of NTHi 86-028NPrpsL, NTHi 86-028NPrpsLM-bM-^HM-^Ffur, NTHi 86-028NPrpsLM-bM-^HM-^FfurM-bM-^HM-^FhrrF, NTHi 86-028NPrpsLM-bM-^HM-^FfurM-bM-^HM-^FhrrF3' strains

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