Project description:Abstract: BRG1 is highly expressed in adult B-cells acute lymphoblastic leukemia and is associated with poor prognosis. High expression of BRG1 promotes the proliferation and resistance to apoptosis of B-ALL cells. BRG1 exerts these functions mainly through activation of the PI3K/AKT signaling pathway. In order to understand the specific regulatory mechanism, we used ChIP-seq to detect the DNA fragments bound by BRG1 in SUP-B15 and Nalm-6 cell lines.
Project description:The acute lymphoblastic leukemia cells lines JM-1, REH, Nalm-27, and SUP-B15 were analyzed for baseline expression of extacellular matrix and adhesion molecules using a pathway focused cDNA microarray (SABiosciences). RNA isolated from the ALL cell lines JM-1, REH, Nalm-27, and SUP-B15 was analyzed using the Extracellular Matrix and Adhesion Molecules Oligo GEArray (SABIosciences).
Project description:The acute lymphoblastic leukemia cells lines JM-1, REH, Nalm-27, and SUP-B15 were analyzed for baseline expression of extacellular matrix and adhesion molecules using a pathway focused cDNA microarray (SABiosciences).
Project description:Four acute lymphoblastic leukemia cell lines SUP-B15, JURKAT, MOLT-3, and CCRF-CEM cells were treated with glucocorticoids such as dexamethasone and prednisolone for six hours before RNA extraction.