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Transcription profiling by array of human mesenchymal stem cells that are highly or lowly migratory

ABSTRACT: To compare the gene expression profile of MSCs harvested from bone marrow in the context of cell migration. Experiment Overall Design: A total of 4 MSCs isolated from different donors were used. Duplicate samples were used for each hybridization. The experiment was repeated twice. CEL files obtained were imported into Partek Genomics suite for analysis. The final processed data were obtained from selecting for genes that have at least 2-fold difference in expression. The gene list was further narrow down to the keywords cell adhesion, chemokine, cytokine, and metalloproteinases.

ORGANISM(S): Homo sapiens

SUBMITTER: Ivy Ho

PROVIDER: E-GEOD-12098 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Matrix metalloproteinase 1 is necessary for the migration of human bone marrow-derived mesenchymal stem cells toward human glioma.

Ho Ivy A W IA Chan Kelly Y W KY Ng Wai-Hoe WH Guo Chang M CM Hui Kam M KM Cheang Philip P Lam Paula Y P PY

Stem cells (Dayton, Ohio) 20090601 6

Human mesenchymal stem cells (MSCs) have increasingly been used as cellular vectors for the delivery of therapeutic genes to tumors. However, the precise mechanism of mobilization remains poorly defined. In this study, MSCs that expressed similar cell surface markers and exhibited multilineage differentiation potentials were isolated from various donors. Interestingly, different MSC isolates displayed differential migration ability toward human glioma cells. We hypothesized that distinct molecul ...[more]

PMID: 19489099

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