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MRNA amount analysis of W303 hog1 mutant strain subjected to osmotic stress

ABSTRACT: mRNA amount (RA) analysis of W303 hog1 mutant yeast strain growing in exponential phase in YPD subjected to osmotic stress Keywords: Time course Transcriptomic analysis of three independent replicates the yeast strain growing in exponential phase. Each time point replicate has been hybridized on a different macroarray (F18-F24).

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin

PROVIDER: E-GEOD-13099 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.

Romero-Santacreu Lorena L Moreno Joaquín J Pérez-Ortín José E JE Alepuz Paula P

RNA (New York, N.Y.) 20090415 6

Hyperosmotic stress yields reprogramming of gene expression in Saccharomyces cerevisiae cells. Most of this response is orchestrated by Hog1, a stress-activated, mitogen-activated protein kinase (MAPK) homologous to human p38. We investigated, on a genomic scale, the contribution of changes in transcription rates and mRNA stabilities to the modulation of mRNA amounts during the response to osmotic stress in wild-type and hog1 mutant cells. Mild osmotic shock induces a broad mRNA destabilization; ...[more]

PMID: 19369426

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