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AURATUS goldfish environmental genomics projects: SKF 38393 in the hypothalamus

ABSTRACT: Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline or SKF 38393 (40 µg/g) dissolved in 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 40 ug/g SKF 38393 hybridized against a common vehicle control. Slides 9, 19, 33, 44.

ORGANISM(S): Carassius auratus

SUBMITTER: Jason Popesku

PROVIDER: E-GEOD-14607 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.

Popesku Jason T JT Martyniuk Christopher J CJ Denslow Nancy D ND Trudeau Vance L VL

PloS one 20100820 8

<h4>Background</h4>Dopamine (DA) is a major neurotransmitter playing an important role in the regulation of vertebrate reproduction. We developed a novel method for the comparison of transcriptomic and proteomic data obtained from in vivo experiments designed to study the neuroendocrine actions of DA.<h4>Methods and findings</h4>Female goldfish were injected (i.p.) with DA agonists (D1-specific; SKF 38393, or D2-specific; LY 171555) and sacrificed after 5 h. Serum LH levels were reduced by 57% a ...[more]

PMID: 20808832

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