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AURATUS goldfish environmental genomics projects: sulpiride in the hypothalamus

ABSTRACT: Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 6, 15, 28, 44.

ORGANISM(S): Carassius auratus

SUBMITTER: Jason Popesku

PROVIDER: E-GEOD-15706 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Meta-type analysis of dopaminergic effects on gene expression in the neuroendocrine brain of female goldfish.

Popesku Jason T JT Martyniuk Christopher J CJ Trudeau Vance L VL

Frontiers in endocrinology 20121102

Dopamine (DA) is a major neurotransmitter important for neuroendocrine control and recent studies have described genomic signaling pathways activated and inhibited by DA agonists and antagonists in the goldfish brain. Here we perform a meta-type analysis using microarray datasets from experiments conducted with female goldfish to characterize the gene expression responses that underlie dopaminergic signaling. Sexually mature, pre-spawning [gonadosomatic index (GSI) = 4.5 ± 1.3%] or sexually regr ...[more]

PMID: 23130016

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AURATUS goldfish environmental genomics projects: sulpiride in the telencephalon

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 12, 26, 37, 48

2013-03-20 | E-GEOD-15705 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SCH 23390 in the hypothalamus

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 40 ug/g SCH 23390 in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 40 ug/g SCH 23390 hybridized against a common vehicle control. Slides 16, 24, 30, 35

2013-03-20 | E-GEOD-15708 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SCH 23390 in the telencephalon

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AURATUS goldfish environmental genomics projects: dopamine antagonists

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Auratus goldfish genome project: Muscimol experiment (6 hour)

Project description:A goldfish brain enriched cDNA combined with a carp microarray (Gracey et al., 2004). Female goldfish were i.p. injected with 1 ug/g muscimol once and after 6 hours, telencephalon dissected and used in microarray analysis. More details on microarray hybridizations and technique used in our laboratory in Martyniuk et al. Gene expression profiling in the brain of male goldfish exposed to 17Î±-EE2(paper under review) Keywords: muscimol, GABA, goldfish, brain 4 arrays using a common control pool and 3 treatment samples. Slides 16, 22, 33, and 50 Slides 22 and 33 are dye swap.

2010-07-01 | E-GEOD-5419 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: quinpirole in the telencephalon

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline OR quinpirole (2 ug/g) dissolved in 0.6% saline. A common reference design was used: 4 independent groups of telencephali from sexually mature female goldfish treated with 2 ug/g quinpirole hybridized against a common vehicle control. Slides 8, 16, 24, 40.

2013-03-20 | E-GEOD-14609 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SKF 38393 in the hypothalamus

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline or SKF 38393 (40 µg/g) dissolved in 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 40 ug/g SKF 38393 hybridized against a common vehicle control. Slides 9, 19, 33, 44.

2011-08-21 | E-GEOD-14607 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SKF 38393 in the telencephalon

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline or SKF 38393 (40 µg/g) dissolved in 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline. A common reference design was used: 4 independent groups of telencephali from sexually mature female goldfish treated with 40 ug/g SKF 38393 hybridized against a common vehicle control. Slides 7, 13, 30, 47.

2013-03-20 | E-GEOD-14608 | biostudies-arrayexpress

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