Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression profiling of Ruminococcus flavefaciens FD-1 with growth on cellulose or cellobiose

ABSTRACT: This study compares growth of Ruminococcus flavefaciens FD-1 with cellulose or cellobiose as the carbohydrate substrate. Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides. Identifying the major enzyme types involved in plant cell wall degradation is essential for gaining a better understanding of the cellulolytic capabilities of this organism as well as highlighting potential enzymes for application to improvement of livestock nutrition and for conversion of cellulosic biomass to liquid fuels. These results show that the growth substrate drives expression of enzymes predicted to be involved in carbohydrate metabolism as well as expression and assembly of key cellulosomal enzyme components. 1 species (Ruminococcus flavefaciens FD_1), 2 conditions (cellulose, cellobiose), 4 biological replicates. Direct design with biological dye swap.

ORGANISM(S): Ruminococcus flavefaciens FD-1

SUBMITTER: Sadanari Jindou

PROVIDER: E-GEOD-15916 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Diversity and strain specificity of plant cell wall degrading enzymes revealed by the draft genome of Ruminococcus flavefaciens FD-1.

Berg Miller Margret E ME Antonopoulos Dionysios A DA Rincon Marco T MT Band Mark M Bari Albert A Akraiko Tatsiana T Hernandez Alvaro A Thimmapuram Jyothi J Henrissat Bernard B Coutinho Pedro M PM Borovok Ilya I Jindou Sadanari S Lamed Raphael R Flint Harry J HJ Bayer Edward A EA White Bryan A BA

PloS one 20090814 8

<h4>Background</h4>Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides. Identifying the major enzyme types involved in plant cell wall degradation is essential for gaining a better understanding of the cellulolytic capabilities of this organism as well as highlighting potential enzymes for application in improvement of li ...[more]

PMID: 19680555

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Dataset Information

Expression profiling of Ruminococcus flavefaciens FD-1 with growth on cellulose or cellobiose

Publications

Diversity and strain specificity of plant cell wall degrading enzymes revealed by the draft genome of Ruminococcus flavefaciens FD-1.

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