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Transcription profiling of human BE(2)-C neuronal responses to type I IFN stimulation and WEEV infection

ABSTRACT: This SuperSeries is composed of the following subset Series:; GSE16450: Human BE(2)-C neuronal responses to type I IFN stimulation; GSE16451: Human BE(2)-C neuronal responses to WEEV infection Experiment Overall Design: Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: David Miller

PROVIDER: E-GEOD-16452 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human neuronal cells possess functional cytoplasmic and TLR-mediated innate immune pathways influenced by phosphatidylinositol-3 kinase signaling.

Peltier Daniel C DC Simms Allison A Farmer Jocelyn R JR Miller David J DJ

Journal of immunology (Baltimore, Md. : 1950) 20100514 12

Innate immune pathways are early defense responses important for the immediate control and eventual clearance of many pathogens, where signaling is initiated via pattern recognition receptor (PRR)-mediated events that occur in a ligand- and cell-type specific manner. Within CNS neurons, innate immune pathways are likely crucial to control pathogens that target these essential yet virtually irreplaceable cells. However, relatively little is known about the induction and regulation of neuronal PRR ...[more]

PMID: 20483728

Similar Datasets

Transcription profiling of human BE(2)-C neuronal responses to type I IFN stimulation

Project description:Human neuronal differentiation alters responsiveness to innate immune stimuli and virus infections. We used microarrays to examine the transcriptional responses of the human BE(2)-C neuroblastoma cell line to retinoic acid-induced differentiation and type I IFN stimulation. Experiment Overall Design: Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks, incubated with universal type I IFN (IFNa-A/D) for 6 or 12 h, and Affymetrix Human Genome Array U133 Plus 2.0 chips were used to analyze transcript levels.

2009-07-12 | E-GEOD-16450 | biostudies-arrayexpress

Transcription profiling of human BE(2)-C neuronal responses to WEEV infection

Project description:Human neuronal differentiation alters responsiveness to innate immune stimuli and virus infections. We used microarrays to examine the transcriptional responses of the human BE(2)-C neuroblastoma cell line to infection with western equine encephalitis virus (WEEV). Experiment Overall Design: Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks and infected with WEEV at an MOI=10 for 6 h, and Affymetrix Human Genome Array U133 Plus 2.0 chips were used to analyze transcript levels.

2009-07-12 | E-GEOD-16451 | biostudies-arrayexpress

Expression data from 205432- or 206381-treated BE(2)-C cells

Project description:Analysis of transcription response of undifferentiated human BE(2)-C neuronal cells to stimulation with novel indole-2-carboxamide antivirals 205432 or 206381. Independent sets of BE(2)-C cells were treated with 25 uM of either 205432 or 206381, or DMSO as control. Total RNA was harvested at 24 h after stimulation and analyzed with Affymetrix U133 Plus 2.0 chips.

2013-10-31 | E-GEOD-51909 | biostudies-arrayexpress

Expression data from WEEV infected BE(2)-C/m cells

Project description:Analysis of transcription response of differentiated human BE(2)-C/m neuronal cells to infection with WEEV at 12 h and 24 h after infection. Two independent sets of BE(2)-C/m cells uninfected or infected with WEEV at an MOI=10 and harvested at 12 h or 24 h after infection. Total RNA was harvested analyzed with Affymetrix U133 Plus 2.0 chips.

2013-11-23 | E-GEOD-52659 | biostudies-arrayexpress

Transcription profiling of Drosophila cells to FHV RNA1 replicon expression (replicon experiment)

Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to Flock House virus (FHV) replicon induction. Experiment Overall Design: Cultured S2 cells stably transfected with either a control replicon (pS2F1fs) or an FHV RNA1 replicon (pS2F1) were induced with 1 mM copper and we measured global transcript levels at 18 h after induction using Affymetrix Drosophila Genome 2.0 microarray chips.

2009-04-11 | E-GEOD-15467 | biostudies-arrayexpress

Expression data from antimycin A-treated BE(2)-C cells

Project description:Analysis of transcription response of undifferentiated human BE(2)-C neuronal cells to stimulation with purified antimycin A1a or unfractionated commercially available antimycin A (Sigma A8674). Three independent sets of BE(2)-C cells were treated DMSO control or 200 nM of either purified antimycin A1a or unfractionated antimycin A. Total RNA was harvested at 24 h after stimulation and analyzed with Affymetrix U133 Plus 2.0 chips.

2013-02-22 | E-GEOD-44541 | biostudies-arrayexpress

Transcriptional response of Drosophila cells to FHV infection (infection experiment)

Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to infection with Flock House virus (FHV). Experiment Overall Design: Cultured S2 cells were infected with FHV at an MOI of 10 and we measured global transcript levels at 12 h after infection compared to control mock infected cells using Affymetrix Drosophila Genome 1.0 microarray chips.

2009-04-11 | E-GEOD-15466 | biostudies-arrayexpress

Transcription profiling of Drosophila cells response to FHV RNA replication

Project description:This SuperSeries is composed of the following subset Series:; GSE15466: Transcriptional response of Drosophila cells to FHV infection (infection experiment); GSE15467: Transcriptional response of Drosophila cells to FHV RNA1 replicon expression (replicon experiment) Experiment Overall Design: Refer to individual Series

2009-04-10 | E-GEOD-15469 | biostudies-arrayexpress

Expression data for PAR-1-positive and -negative melanoma cell lines

Project description:PAR-1 is known to be involved in the transition from non-metastatic to metastatic melanoma. We sought to determine the downstream target genes regulated by PAR-1 to determine how PAR-1 is contributing to the metastatic melanoma phenotype. The A375SM metastatic melanoma cell line was subjected to stable transduction of lentiviral-delivered small hairpin RNA (shRNA) targeting PAR-1. As a control, a non-targeting shRNA sequence (no homology to any human gene) was also transduced into A375SM cells.

2010-09-22 | E-GEOD-24259 | biostudies-arrayexpress

Histone acetylation dependent microarray analysis uncovers a role for Tip60 HAT activity in nervous system function and general metabolism

Project description:Tip60 is a key histone acetyltransferase (HAT) enzyme that plays a central role in diverse biological processes critical for general cell function, however the chromatin-mediated cell-type specific developmental pathways that are dependent exclusively upon the HAT activity of Tip60 remain to be explored. Here, we investigate the role of Tip60 HAT activity in transcriptional control during multicellular development, in vivo by examining genome-wide changes in gene expression in a Drosophila model system specifically depleted for endogenous dTip60 HAT function. We show that amino acid residue E431 in the catalytic HAT domain of dTip60 is critical for the acetylation of endogenous histone H4 in our fly model in vivo, and demonstrate that dTip60 HAT activity is essential for multicellular development. Moreover, our results uncover a novel role for Tip60 HAT activity in controlling neuronal specific gene expression profiles essential for nervous system function as well as a central regulatory role for Tip60 HAT function in general metabolism. Drosophila larvae ubiquitously expressing either our dTIP60 HAT specific mutant or an additional copy of wild type dTIP60 were selected along with age-matched wild type controls for RNA extraction. Two samples, each containing a pool of thirty-five staged whole larvae, were collected from each respective genotypic cross, and were used for hybridization on a separate Affymetrix microarray.

2010-11-29 | E-GEOD-25635 | biostudies-arrayexpress

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