Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-chip of Spo14 and Mvp1


ABSTRACT: Genome-wide localization of two new RNA pol II interacting proteins. We use C-LYTAG to map binding sites of Spo14 and Mvp1 along the S. cerevisiae genome. Two biological replicates of each experiment were done. Keywords: ChIp-chip Two independent biological replicates of each IP. We used three macroarrays: V5R2-L1-20, V5R2-L1-21 and V5R2-L1-22 that were hybridized with Whole Cell Extract (WCE) and then with two biological samples of immunoprecipitated (IP) Mvp1 (V5R2-L1-20 and V5R2-L1-21) and Spo14 (V5R2-L1-20 and V5R2-L1-22)

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin 

PROVIDER: E-GEOD-16905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation.

García-López M Carmen MC   Pelechano Vicent V   Mirón-García M Carmen MC   Garrido-Godino Ana I AI   García Alicia A   Calvo Olga O   Werner Michel M   Pérez-Ortín José E JE   Navarro Francisco F  

Genetics 20110927 4


RNA polymerase (pol) II establishes many protein-protein interactions with transcriptional regulators to coordinate different steps of transcription. Although some of these interactions have been well described, little is known about the existence of RNA pol II regions involved in contact with transcriptional regulators. We hypothesize that conserved regions on the surface of RNA pol II contact transcriptional regulators. We identified such an RNA pol II conserved region that includes the majori  ...[more]

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