Unknown,Transcriptomics,Genomics,Proteomics

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Expression profile of contrasting maize genotypes grown on acid and control soil (root tips)


ABSTRACT: Aluminum toxicity is one of the major limiting factors for many crops worldwide. The primary symptom of Al toxicity syndrome is the inhibition of root growth, leading to poor water and nutrient absorption. The causes of this inhibition are still elusive, with several biochemical pathways being affected and with a significant variation between species. Most of the work done so far to investigate the genes responsible for Al tolerance used hydroponic culture. Here we evaluated plant responses using soil as substrate, which is a condition closer to the field reality. We used Affymetrix chips to reveal the transcriptional changes of two maize genotypes contrasting for Al tolerance. Root tips from Cat100-6 (Al-tolerant) and S1587-17 (Al-sensitive) maize genotypes were harvested after growing on acid or control soil for 1 or 3 days. Total RNA was extracted and hybridized to Affymetrix GeneChip Maize Genome Array. Three biological replicates were used for each sample.

ORGANISM(S): Zea mays

SUBMITTER: Marcelo Menossi 

PROVIDER: E-GEOD-21070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional profile of maize roots under acid soil growth.

Mattiello Lucia L   Kirst Matias M   da Silva Felipe R FR   Jorge Renato A RA   Menossi Marcelo M  

BMC plant biology 20100909


<h4>Background</h4>Aluminum (Al) toxicity is one of the most important yield-limiting factors of many crops worldwide. The primary symptom of Al toxicity syndrome is the inhibition of root growth leading to poor water and nutrient absorption. Al tolerance has been extensively studied using hydroponic experiments. However, unlike soil conditions, this method does not address all of the components that are necessary for proper root growth and development. In the present study, we grew two maize ge  ...[more]

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