Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression variation in Y-substitution lines in different genetic backgrounds of Drosophila melanogaster

ABSTRACT: We studied Y chromosomes from two geographically diverse populations of Drosophila melanogaster, and show that these Y chromosomes differentially modify the expression of hundreds of autosomal and X-linked genes. Genes showing Y-linked regulatory variation also show an association with immune response and pheromone detection, as well as proximity to the X chromosome euchromatin-heterochromatin boundary. 4-6 dual channel arrays per background, with males carrying two Y geographically diverse chromosomes competitively hybridized to each array. Dye-swap design was used.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Pan-Pan Jiang

PROVIDER: E-GEOD-21587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Y not a dead end: epistatic interactions between Y-linked regulatory polymorphisms and genetic background affect global gene expression in Drosophila melanogaster.

Jiang Pan-Pan PP Hartl Daniel L DL Lemos Bernardo B

Genetics 20100615 1

The Y chromosome, inherited without meiotic recombination from father to son, carries relatively few genes in most species. This is consistent with predictions from evolutionary theory that nonrecombining chromosomes lack variation and degenerate rapidly. However, recent work has suggested a dynamic role for the Y chromosome in gene regulation, a finding with important implications for spermatogenesis and male fitness. We studied Y chromosomes from two populations of Drosophila melanogaster that ...[more]

PMID: 20551438

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Project description:Copy number variants (CNVs) reshape gene structure, modulate gene expression, and contribute to significant phenotypic variation. Previous studies have revealed CNV patterns in natural populations of Drosophila melanogaster and suggested that selection and mutational bias shape genomic patterns of CNV. While previous CNV studies focused on heterogeneous strains, here we established a number of second-chromosome substitution lines to uncover CNV characteristics when homozygous. The percentage of genes harboring CNVs is higher than found in previous studies. More CNVs are detected in homozygous than heterozygous substitution strains, suggesting the comparative genomic hybridization arrays underestimate CNV owing to heterozygous masking. We incorporated previous gene expression data collected from some of the same substitution lines to investigate relationships between CNV gene dosage and expression. Most genes present in CNVs show no evidence of increased or diminished transcription, and the fraction of such dosage-insensitive CNVs is greater in heterozygotes. More than 70% of the dosage-sensitive CNVs are recessive with undetectable effects on transcription in heterozygotes. A deficiency of singletons in recessive dosage-sensitive CNVs supports the hypothesis that most CNVs are subject to negative selection. On the other hand, relaxed purifying selection might account for the higher number of protein-protein interactions in dosage insensitive CNVs than in dosage-sensitive CNVs. Dosage-sensitive CNVs that are up-regulated and down-regulated coincide with copy number increases and decreases. Our results help clarify the relation between CNV dosage and gene expression in the D. melanogaster genome. To determine copy number variation, the genomic DNA from five homozygous and two heterozygous second chromosome substitution lines were extracted and compared to another second chromosome substitution line. Gene expression levels can be referred to at Series GSE12191 (Lemos et al. (2008) PMID:18791071).

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Dataset Information

Gene expression variation in Y-substitution lines in different genetic backgrounds of Drosophila melanogaster

Publications

Y not a dead end: epistatic interactions between Y-linked regulatory polymorphisms and genetic background affect global gene expression in Drosophila melanogaster.

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