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An integrated model of stress-dependent gene expression in yeast

ABSTRACT: Microarray timecourses followed the gene expression response of Saccharomyces cereviciae to osmotic shock, over 4 hours. Data was compared to timecourses of quantitative proteomic data to understand the dynamic relationship between RNA and protein The dynamic genomic expression response to osmotic shock (0.7M NaCl) was measured in Saccharomyces cereviciae, over the course of 4 hours in biological triplicate. Six timepoints were taken for each time course, for a total of 18 tiled-genomic Nimblegen arrays against the S288c yeast genome.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Audrey Gasch

PROVIDER: E-GEOD-23798 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A dynamic model of proteome changes reveals new roles for transcript alteration in yeast.

Lee M Violet MV Topper Scott E SE Hubler Shane L SL Hose James J Wenger Craig D CD Coon Joshua J JJ Gasch Audrey P AP

Molecular systems biology 20110719

The transcriptome and proteome change dynamically as cells respond to environmental stress; however, prior proteomic studies reported poor correlation between mRNA and protein, rendering their relationships unclear. To address this, we combined high mass accuracy mass spectrometry with isobaric tagging to quantify dynamic changes in ~2500 Saccharomyces cerevisiae proteins, in biological triplicate and with paired mRNA samples, as cells acclimated to high osmolarity. Surprisingly, while transcrip ...[more]

PMID: 21772262

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