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TR heat-shock

ABSTRACT: Within a GRO experiment, samples for Transcription rate (TR) analysis were taken at 0, 4, 11, 16, 26 and 40 minutes after heat stress (from 25ºC to 37ºC) and subjected to run-on. Analysis was done in filters also used for RA analysis of aliquots from the same cultures. The analysis includes 3 repeats of stress treatment of the W3030-1a strain. The same times were used for sampling in each repeat of the experiment. A single genomic DNA hybridization on every one of the filters is used for normalization between gene probes.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin

PROVIDER: E-GEOD-24483 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Heat shock response in yeast involves changes in both transcription rates and mRNA stabilities.

Castells-Roca Laia L García-Martínez José J Moreno Joaquín J Herrero Enrique E Bellí Gemma G Pérez-Ortín José E JE

PloS one 20110225 2

We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25 °C to 37 °C. Using an established mathematical algorithm, theoretical mRNA decay rates have also been calculated from the experimental data. We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO p ...[more]

PMID: 21364882

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