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Topo mutants psoralen photobinding

ABSTRACT: We present a DNA array-based method for the genome-wide analysis of Psoralen photobinding in Saccharomyces cerevisiae cells. PB was found reduced in subtelomeric regions and increased in the subset of genes with smallest nucleosomal density. The analysis includes 3 repeats of the wild type strain (JCW25) at 30ÂºC and 3 more at 37ÂºC, 3 repeats of the double top1 (delta) top2ts mutant at 37ÂºC and 3 repeats of naked genomic DNA treated with trimethyl psoralen (TMP) in the same way to be used as a control of unspecific binding.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin

PROVIDER: E-GEOD-16758 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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