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Transcription profiling of rat primary cortical neurons exposed to anesthetics

ABSTRACT: Inhaled anesthetics produce many effects and bind to a large number of brain proteins, but it is not yet clear if this is accompanied by widespread changes in gene expression of the biological targets. Such changes in expression might implicate functionally important targets from the large pool of binding targets. Isolated primary cortical neurons were exposed to anesthetics and DNA oligonucleotide microarrays were used to detect and quantify transcriptional changes in neuronal tissue. Experiment Overall Design: Primary cortical neurons were treated with 1MAC, 3MAC Halothane and 3MAC Isoflurane, together with no drug control. Pool no drug control was used as Cy3 channel in all chips. The above drug treated and individual control samples were used in Cy5 channel.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Roderic Eckenhoff

PROVIDER: E-GEOD-2982 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rat brain DNA transcript profile of halothane and isoflurane exposure.

Pan Jonathan Z JZ Wei Huafeng H Hecker James G JG Tobias John W JW Eckenhoff Roderic G RG Eckenhoff Maryellen Fazen MF

Pharmacogenetics and genomics 20060301 3

Inhaled anesthetics produce many effects and bind to a large number of brain proteins, but it is not yet clear if this is accompanied by widespread changes in gene expression of the biological targets. Such changes in expression might implicate functionally important targets from the large pool of binding targets. Both rats and isolated primary cortical neurons were exposed to anesthetics and DNA oligonucleotide microarrays were used to detect and quantify transcriptional changes in neural tissu ...[more]

PMID: 16495776

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