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Gene expression in cellular compartments of Aplysia sensory neurons

ABSTRACT: Whole genome transcriptional profiling is used to compare ESTs found in cell bodies and processes of Aplysia sensory neurons RNA samples derived from cell bodies or processes of Aplysia single cultured sensory neurons were hybridized to custom Aplysia EST microarrays. Two-condition experiment; four biological replicates for each condition were reciprocally hybridized on each two-color array

ORGANISM(S): Aplysia californica

SUBMITTER: Sergey Kalachikov

PROVIDER: E-GEOD-30388 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A strategy to capture and characterize the synaptic transcriptome.

Puthanveettil Sathyanarayanan V SV Antonov Igor I Kalachikov Sergey S Rajasethupathy Priyamvada P Choi Yun-Beom YB Kohn Andrea B AB Citarella Mathew M Yu Fahong F Karl Kevin A KA Kinet Maxime M Morozova Irina I Russo James J JJ Ju Jingyue J Moroz Leonid L LL Kandel Eric R ER

Proceedings of the National Academy of Sciences of the United States of America 20130415 18

Here we describe a strategy designed to identify RNAs that are actively transported to synapses during learning. Our approach is based on the characterization of RNA transport complexes carried by molecular motor kinesin. Using this strategy in Aplysia, we have identified 5,657 unique sequences consisting of both coding and noncoding RNAs from the CNS. Several of these RNAs have key roles in the maintenance of synaptic function and growth. One of these RNAs, myosin heavy chain, is critical in pr ...[more]

PMID: 23589870

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