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Chromatin immunoprecipitation of human Hela cells grown under serum starved condtions to identify new targets genes for ETS-domain transcription factor Elk-1

ABSTRACT: Chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip) was carried out to identify new target genes for ETS-domain transcription factor Elk-1. Experiment was done in Hela cells under serum starved conditions.

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-1527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Elucidation of the ELK1 target gene network reveals a role in the coordinate regulation of core components of the gene regulation machinery.

Boros Joanna J Donaldson Ian J IJ O'Donnell Amanda A Odrowaz Zaneta A ZA Zeef Leo L Lupien Mathieu M Meyer Clifford A CA Liu X Shirley XS Brown Myles M Sharrocks Andrew D AD

Genome research 20090817 11

Transcription factors play an important role in orchestrating the activation of specific networks of genes through targeting their proximal promoter and distal enhancer regions. However, it is unclear how the specificity of downstream responses is maintained by individual members of transcription-factor families and, in most cases, what their target repertoire is. We have used ChIP-chip analysis to identify the target genes of the ETS-domain transcription factor ELK1. Two distinct modes of ELK1 ...[more]

PMID: 19687146

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Chromatin immunoprecipitation of human HeLa cells grown under serum starved conditions to identify new target genes for ETS-domain transcription factor Elk-1

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