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Role of microRNAs and microRNA machinery in the pathogenesis of Diffuse large B-cell Lymphomas

ABSTRACT: We performed miRNA expression profiling in a series of de novo DLBCLs, transformed DLBCLs and non-neoplastic lymph nodes using a microarray approach. Significant differentially expressed miRNAs among groups were identified using SAM analysis. Agilent microarray platform containing 470 miRNAs was used to determine miRNA expression profiles in 45 DLBCL cases (32 de novo and 13 transformed) and 10 lymph nodes. To validate the microarray platform, the expression levels of selected miRNAs were evaluated using qRT-PCR.

ORGANISM(S): Homo sapiens

SUBMITTER: Stefano Caramuta

PROVIDER: E-GEOD-42906 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma.

Caramuta S S Lee L L Ozata D M DM Akçakaya P P Georgii-Hemming P P Xie H H Amini R-M RM Lawrie C H CH Enblad G G Larsson C C Berglund M M Lui W-O WO

Blood cancer journal 20131011

Deregulation of microRNA (miRNA) expression has been documented in diffuse large B-cell lymphoma (DLBCL). However, the impact of miRNAs and their machinery in DLBCL is not fully determined. Here, we assessed the role of miRNA expression and their processing genes in DLBCL development. Using microarray and RT-qPCR approaches, we quantified global miRNAs and core components of miRNA-processing genes expression in 75 DLBCLs (56 de novo and 19 transformed) and 10 lymph nodes (LN). Differential miRNA ...[more]

PMID: 24121164

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