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Analysis of Rice microRNAs Expression under Drought Stress


ABSTRACT: We used this microarray to monitor rice miRNA expression profile under osmotic stress. Most of rice miRNAs have no significant change. miR-169f showed osmotic-responsive up-regulation. However, the others members of miR-169 family displays no responses to the osmotic stress. Keywords: microRNA,rice,osmotic stress,miR-169 The six selected time points after PEG treatment were 0h, 0.5h, 2h, 6h, 24h and 48h. For each sample, two independent small RNA libraries (biological replications) were generated and two independent reverse transcription/amplifications/hybridizations (technical replications) were performed for each library. Thus, for each sample data was collected from four independent hybridizations.

ORGANISM(S): Oryza sativa

SUBMITTER: Liangfa Ge 

PROVIDER: E-GEOD-5986 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of drought-induced microRNAs in rice.

Zhao Botao B   Liang Ruqiang R   Ge Liangfa L   Li Wei W   Xiao Huasheng H   Lin Hongxuan H   Ruan Kangcheng K   Jin Youxin Y  

Biochemical and biophysical research communications 20070112 2


MicroRNAs (miRNAs) are a large new class of small non-coding RNAs. To date, hundreds of microRNAs have been identified in plants. MicroRNAs play important roles in post-transcriptional gene regulation by targeting mRNAs for cleavage or repressing translation. To better understand microRNA function, we have used an oligonucleotide microarray to monitor rice (Oryza sativa) microRNA expression profile under drought stress. Two drought-induced microRNAs were identified. Furthermore, miR-169g was con  ...[more]

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