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EQTL Mapping of the Yeast Ethanol Response

ABSTRACT: In a previous study, we identified extensive natural variation in the response to acute ethanol treatment in three yeast strains: a lab strain derived from the commonly used S288c (DBY8268), vineyard isolate M22, and oak soil strain YPS163. Many expression differences persisted across several modules of co-regulated genes, implicating trans-acting systemic differences in ethanol sensing and/or response. To understand the genetic basis for these expression differences, we performed eQTL mapping analysis of the response to acute ethanol stress in ~100 F2 strains from two crosses: DBY8268x M22 and DBY8268 x YPS163. We measured the gene expression response to acute ethanol stress (5% for 30 min) in 6 replicates for the parental strains, and in biological duplicate for the eQTL mapping population. Dye swaps were performed in the replicates to control for dye-specific effects.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jeffrey Lewis

PROVIDER: E-GEOD-54196 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic architecture of ethanol-responsive transcriptome variation in Saccharomyces cerevisiae strains.

Lewis Jeffrey A JA Broman Aimee T AT Will Jessica J Gasch Audrey P AP

Genetics 20140626 1

Natural variation in gene expression is pervasive within and between species, and it likely explains a significant fraction of phenotypic variation between individuals. Phenotypic variation in acute systemic responses can also be leveraged to reveal physiological differences in how individuals perceive and respond to environmental perturbations. We previously found extensive variation in the transcriptomic response to acute ethanol exposure in two wild isolates and a common laboratory strain of ...[more]

PMID: 24970865

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